SureFood® ALLERGEN Fish
Art. No. S3610
Product info about SureFood® ALLERGEN Fish
Intended use
The SureFood® ALLERGEN Fish is a real-time PCR for the direct, qualitative and / or quantitative detection of specific fish DNA sequences according to directive (EC) 1169/2011. The group of fishes contains about 30000 species. Due to the large number of different species a practical verification of all is impossible. More detailed information is available in the Validation Report.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg fish / kg food sample and a detection system including a standard series is required.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The internal technical verification of instruments was performed on Agilent AriaDx, Bio-Rad CFX96 Dx, R-Biopharm RIDA®CYCLER, Qiagen Rotor-Gene Q and Roche LightCycler® 480 II.
| Specifications | |
|---|---|
| Art. No | S3610 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended. |
| LOD (Detection limit) | The SureFood® ALLERGEN Fish PCR has a limit of detection of ≤ 1 mg / kg using SureFood® PREP Advanced, protocol 1. |
| LOQ (Limit of quantification | 4 mg / kg using SureFood® PREP Advanced, protocol 1 |
| Cross Reactivity. | Cross reactivity was observed with DNA extracts from muscovy duck (Cairina moschata). |
