EuroProxima DNSH
Art. No.: 5091DNSH
Product info
Intended use
EuroProxima DNSH is a competitive enzyme immunoassay for the quantitative detection of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.
General Information
Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolised to 3,5-dinitrosalicyclic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatisation of this metabolite with 2-nitrobenzaldehyde to NPDNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. In accordance with Commission Regulation (EU) 2019/1871 a reference point of action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.
DNSH ELISA is a specific method for the detection of DNSH in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn). The antibody used in this test detects DNSH without derivatisation with 2-nitrobenzaldehyde.
Accessories
Dear customers,
we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/RBNL.
| Specifications | |
|---|---|
| Art. No | 5091DNSH |
| Test format | Microtiter plate with 96 wells (12 strips with 8 wells each) |
| Sample preparation | For mentioned matrices fast and efficient extraction methods are included in the kit description. |
| Incubation time | 1 h (20 - 25 °C) |
| LOD (Detection Limit) | Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg |
| Detection Capability | 0.25 µg/kg |
| Validated matrices | Meat and seafood. |
| Detected analyte | 3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood. |
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
| Files | |
|---|---|
| Instructions | English |
| MSDS | 5091DNSH-SDS-1.zip (English) |