EuroProxima DNSH

Art. No.: 5091DNSH

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Product info

EuroProxima DNSH

Art. No.: 5091DNSH

Intended use

EuroProxima DNSH is a competitive enzyme immunoassay for the quantitative detection of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.

General Information

Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolised to 3,5-dinitrosalicyclic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatisation of this metabolite with 2-nitrobenzaldehyde to NPDNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. In accordance with Commission Regulation (EU) 2019/1871 a reference point of action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.
DNSH ELISA is a specific method for the detection of DNSH in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn). The antibody used in this test detects DNSH without derivatisation with 2-nitrobenzaldehyde.


Art. No5091DNSH
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each)
Sample preparationFor mentioned matrices fast and efficient extraction methods are included in the kit description.
Incubation time1 h (20 - 25 °C)
LOD (Detection Limit)Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg
Detection Capability0.25 µg/kg
Validated matrices

Meat and seafood.

Detected analyte

3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.

EvaluationMicrotiter plate spectrophotometer (450 nm)