Art. No.: 5091DNSH
EuroProxima DNSH is a competitive enzyme immunoassay for the quantitative detection of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.
Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolised to 3,5-dinitrosalicyclic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatisation of this metabolite with 2-nitrobenzaldehyde to NPDNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. In accordance with Commission Regulation (EU) 2019/1871 a reference point of action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.
DNSH ELISA is a specific method for the detection of DNSH in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn). The antibody used in this test detects DNSH without derivatisation with 2-nitrobenzaldehyde.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||1 h (20 - 25 °C)|
|LOD (Detection Limit)||Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg|
|Detection Capability||0.25 µg/kg|
Meat and seafood.
3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.
|Evaluation||Microtiter plate spectrophotometer (450 nm)|