Art. No.: R3740
RIDASCREEN® DNSH (Art. No. R3740) is a competitive enzyme immunoassay for the quantitative analysis of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn).
Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolized to 3,5-dinitrosalicylic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatization of this metabolite with 2-nitrobenzaldehyde to NP-DNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. The antibody used in this test detects DNSH without derivatization with 2-nitrobenzaldehyde. In accordance with Commission Regulation (EU) 2019/1871 a reference point for action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.
we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||Homogenization and extraction|
|Incubation time||1 h (20 - 25 °C)|
|LOD (Detection Limit)||Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg|
|Detection Capability||0.25 µg/kg|
Meat, fish and shellfish.
3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.
|Evaluation||Microtiter plate spectrophotometer (450 nm)|