Art. No.: R3740

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Art. No.: R3740

Intended use

RIDASCREEN® DNSH (Art. No. R3740) is a competitive enzyme immunoassay for the quantitative analysis of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn).

General Information

Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolized to 3,5-dinitrosalicylic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatization of this metabolite with 2-nitrobenzaldehyde to NP-DNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. The antibody used in this test detects DNSH without derivatization with 2-nitrobenzaldehyde.
In accordance with Commission Regulation (EU) 2019/1871 a reference point for action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.

Art. NoR3740
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each)
Sample preparationHomogenization and extraction
Incubation time1 h (20 - 25 °C)
LOD (Detection Limit)Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg
Detection Capability0.25 µg/kg
Validated matrices

Meat, fish and shellfish.

Detected analyte

3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.

EvaluationMicrotiter plate spectrophotometer (450 nm)