Art. No.: R3740

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Art. No.: R3740

Intended use

RIDASCREEN® DNSH (Art. No. R3740) is a competitive enzyme immunoassay for the quantitative analysis of nifursol metabolite 3,5-dinitrosalicylic acid hydrazide (DNSH) in meat (poultry, pork, beef) and seafood (fish, shrimp, and prawn).

General Information

Nifursol is a nitrofuran antibiotic banned as a feed additive in the European Union and other countries. Nifursol is metabolized to 3,5-dinitrosalicylic acid hydrazide (DNSH) in living organisms. DNSH is a marker for the detection of illegal use of nifursol in animal husbandry. The detection of DNSH by LC-MS/MS requires derivatization of this metabolite with 2-nitrobenzaldehyde to NP-DNSH, like other nitrofuran metabolites such as SEM, AHD, AMOZ and AOZ. The antibody used in this test detects DNSH without derivatization with 2-nitrobenzaldehyde. In accordance with Commission Regulation (EU) 2019/1871 a reference point for action (RPA) of 0.5 µg/kg for DNSH and other nitrofuran metabolites applies from 28 November 2022.

Art. NoR3740
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each)
Sample preparationHomogenization and extraction
Incubation time1 h (20 - 25 °C)
LOD (Detection Limit)Meat approx. 0.13 µk/kg (ppb), fish and shellfish approx. 0.15 µg/kg
Detection Capability0.25 µg/kg
Validated matrices

Meat, fish and shellfish.

Detected analyte

3,5-Dinitrosalicylic acid hydrazide (DNSH) in meat and seafood.

EvaluationMicrotiter plate spectrophotometer (450 nm)