SureFood® ALLERGEN Brazil Nut
Art. No.: S3617
The SureFood® ALLERGEN Brazil Nut is a real-time PCR for the direct, qualitative and / or quantitative detection of specific brazil nut (Bertholletia excelsa) DNA sequences according to directive (EC) 1169/2011.
For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg brazil nut / kg food sample and a detection system including a standard series is required.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.|
|LOD (Detection Limit)||The SureFood® ALLERGEN Brazil Nut PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.|
|LOQ (Limit of quantification|
1 mg / kg using SureFood® PREP Advanced, protocol 1