SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

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SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

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Intended use

The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya illinoinensis) DNA sequences according to directive (EC) 1169/2011.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Aria Dx, Bio-Rad CFX96 Dx, Roche LightCycler® 480 II and R-Biopharm RIDACYCLER.

Accessories

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com.

Specifications
Art. NoS3403
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
Cross Reactivity.

For the Pecan real-time PCR system cross reactivity was observed with DNA extracts from shagbark hickory (Carya Ovata).