SureFast® Foodborne Pathogens 4plex
Art. No.: F5175
The SureFast® Foodborne Pathogens 4plex is a real-time PCR for for the direct, qualitative detection and differentiation of Escherichia coli virulence factors (stx1 [subtype a-d], stx2 [subtype a-g]), Listeria monocytogenes and Salmonella spp..
Each reaction contains an internal amplification control.
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time.
The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Agilent AriaDx and Agilent Mx3005P.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Bacteria is recommended.|
|LOD (Detection Limit)||The SureFast® Foodborne Pathogens 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|