SureFast® Foodborne Pathogens 4plex

Art. No.: F5175

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Product info

SureFast® Foodborne Pathogens 4plex

Art. No.: F5175

Intended use

The SureFast® Foodborne Pathogens 4plex is a real-time PCR for for the direct, qualitative detection and differentiation of Escherichia coli virulence factors (stx1 [subtype a-d], stx2 [subtype a-g]), Listeria monocytogenes and Salmonella spp..

Each reaction contains an internal amplification control.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time.
The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Agilent AriaDx and Agilent Mx3005P.


Art. NoF5175
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Bacteria is recommended.
LOD (Detection Limit)The SureFast® Foodborne Pathogens 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.