SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

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Product info

SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

Intended use

The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya illinoinensis) DNA sequences according to directive (EC) 1169/2011.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Aria Dx, Bio-Rad CFX96 Dx, Roche LightCycler® 480 II and R-Biopharm RIDACYCLER.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.

Specifications
Art. NoS3403
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
Cross Reactivity.

For the Pecan real-time PCR system cross reactivity was observed with DNA extracts from shagbark hickory (Carya Ovata).