Intended use:

The multiplex test detects DNA of macadamia, brazil nut and pecan according to directive (EC) 1169/2011. Each reaction contains an internal amplification control.

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. Internal validation was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent Aria Dx, Bio-Rad CFX96, Roche LightCycler® 480 II2, Roche cobas z 480 Analyzer2 and Qiagen Rotorgene Q.

Accessories:

Specifications
Art. No. S3403
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.
LOD (Detection limit) The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan+IAC PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1. The limit of detection was determined using the SureFood® QUANTARD Allergen 40 (matrix: corn flour).
Cross reactivity

For the Pecan real-time PCR system cross reactivity was observed with DNA extracts from shagbark hickory (Carya Ovata).

Files
InstructionsGerman/English
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