SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC
Art. No.: S3403
Product info
Intended use
The multiplex real-time PCR test detects DNA of macadamia, brazil nut and pecan according to directive (EC) 1169/2011 qualitatively. Each reaction contains an internal amplification control (IAC).
General Information
For DNA preparation, the use of SureFood® PREP Advanced (S1053), protocol 1, is recommended.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for the detection of four fluorescence emissions at 510 nm, 580 nm, 610 nm and 660 nm (FAM, VIC/HEX, ROX and Cy5) at the same time. Internal validation was performed on Applied Biosystems 7500, Agilent Mx3005P, Agilent Aria Dx, Bio-Rad CFX96, Roche LightCycler® 480 II2, Roche cobas z 480 Analyzer2 and Qiagen Rotorgene Q.
Accessories
| Specifications | |
|---|---|
| Art. No | S3403 |
| Test format | 100 reactions |
| LOD (Detection Limit) | ≤ 0.4 mg / kg |
| Cross Reactivity. | For the Pecan real-time PCR system cross reactivity was observed with DNA extracts from shagbark hickory (Carya Ovata). |
| Evaluation | Real-time PCR instrument with four detection channels (510 nm, 580 nm, 610 nm and 660 nm) |
