SureFood® GMO SCREEN P35S:BAR Rice

Art. No.: S2022

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SureFood® GMO SCREEN P35S:BAR Rice

Art. No.: S2022
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Intended use

The SureFood® GMO SCREEN P35S:BAR Rice is a real-time PCR for the direct, qualitative detection of a specific P35S:BAR constructDNA sequence of P35S:BAR. The LibertyLink 601 (LL601 – OECD unique identifier BCS-OSØØ3-7) Rice and the LibertyLink 62 (LL62 – OECD unique identifier ACS-OSØØ2-5) Rice contains this genetically construct but it is also present in other GMOs as StarLink Corn and Bt176 Corn. Additionally, the kit contains a reference PCR system for the detection of Rice.

The specific detection is according to the validated method of the European Commission (“Report on the verification of a construct-specific detection method for identification of Rice GM-events containing P35S:BAR using a real-time PCR assay” from the Joint Research Centre, the Community Reference Laboratory).

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, LTF MyGo Pro, Agilent AriaDx and Agilent Mx3005P.

Accessories

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com.

Specifications
Art. NoS2022
Test format100 reactions, whereas 50 reactions are used for detection of the reference gene.
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
LOD (Detection Limit)The P35S:BAR PCR has a limit of detection of ≤ 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed Rice grain. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.