
SureFast® Fecal Screen 4plex
Art. No. F5504
Analytes
Product info about SureFast® Fecal Screen 4plex
Intended use
The SureFast® Fecal Screen 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific DNA sequences of Enterobacteriaceae, Enterococci, Escherichia coli in water.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical verification of instruments was performed on Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Bio-Rad CFX96Dx, R-Biopharm RIDA®CYCLER and Agilent AriaDx.
Specifications | |
---|---|
Art. No | F5504 |
Test format | 100 reactions |
Sample preparation | For DNA-preparation the use of SureFast® PREP Aqua (Art. Nr. F1023) is recommended. |
LOD (Detection Limit) | The SureFast® Fecal Screen 4plex real-time PCR has a limit of detection of < 5 DNA copies. |
Validated matrices | Water portions horse |