EuroProxima Sulfamethazine

Art. No.: 5101SUL

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Analytes

Product info

EuroProxima Sulfamethazine

Art. No.: 5101SUL
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Intended use

EuroProxima Sulfamethazine is a competitive enzyme immunoassay for quantitative analysis of sulfamethazine in urine, tissue, milk and serum/plasma.

General Information

Sulfamethazine (SMZ) (syn. for Sulfadimidine or Sulfamezathine or 2-sulfanylamido4,6-dimethylpyrimidine) is widely used for therapeutic and prophylactic purposes in animal food production. Sulfonamides which are retained in food could result in allergic or toxic reactions in for these compounds sensitive consumers. Also, there are general concerns that the widespread use of antibiotics could contribute to antibiotic resistance in pathogenic organisms. Normally, tissue residues in animals are controlled by withdrawing the drug from feed before slaughter. The concentrations of the sulfonamides are then presumed to deplete to less than the maximum residue level (MRL). However, due to contaminated food or failure to observe the withdrawal period, a number of animals are reaching slaughterhouses with a substantially excessive amount of drugs still present in their tissues. SMZ is partially metabolised in animals. Main metabolite in horses is 5-hydroxy-SMZ, in cows, calves, goats, turtles and snails it is 6-hydroxymethyl-SMZ. In man and pigs the main metabolite is N4-acetyl-SMZ. The amount of SMZ metabolised depends on many factors, i.e. type of animal, dose and time after administration. In the E.C. and the U.S.A. the MRL for sulfa residues in tissue and milk is 100 µg/kg. For the determination of Sulfonamides, analytical procedures based on HPLC have been developed. However, such methods are expensive and time consuming. This ELISA detects SMZ and N4-acetyl-SMZ in urine, tissue, milk and plasma with a fast and simple sample preparation.

Specifications
Art. No5101SUL
Test formatMicrotiter plate with 96 wells (12 strips with 8 wells each).
Sample preparationFor mentioned matrices fast and efficient extraction methods are included in the kit description.
Incubation time1 h 30 min
LOD (Detection Limit)Urine (3 μg/L (ppb)), tissue (3 μg/kg), milk (8 μg/L), serum/plasma (1 μg/L)
Validated matrices

Urine, tissue, milk and serum/plasma.

Detected analyte

Sulfamethazine in urine, tissue, milk and serum/plasma.

EvaluationMicrotiter plate spectrophotometer (450 nm)
Files
InstructionsEnglish
MSDS5101SUL.zip (English)