SureFast® Vibrio 4plex

Art. No.: F5161

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Product info

SureFast® Vibrio 4plex

Art. No.: F5161
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Intended use

The SureFast® Vibrio 4plex is a multiplex real-time PCR for the direct, qualitative detection and differentiation of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus DNA.

Each reaction contains an internal amplification control (IAC).

General Information

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time.

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500 and R-Biopharm RIDA®CYCLER.

Accessories

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.

Specifications
Art. NoF5161
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.
LOD (Detection Limit)The SureFast® Vibrio 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.