Intended use:

The SureFast® Vibrio 4plex is a real-time PCR for the direct, qualitative detection and differentiation of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus DNA.

Each reaction contains an internal amplification control (IAC).

General information:

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time.

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Bio-Rad CFX96, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500 and R-Biopharm RIDA®CYCLER.

Accessories:

Specifications
Art. No. F5161
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.
LOD (Detection limit) The SureFast® Vibrio 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Files
InstructionsGerman/English
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