The SureFast® Vibrio 4plex is a real-time PCR for the direct, qualitative detection and differentiation of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus DNA.
The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of four fluorescence emissions at 522 nm, 553 nm, 610 nm and 670 nm (FAM, VIC, ROX and Cy5) at the same time.
The technical validation of instruments was performed on Agilent Mx3005P, BioRad CFX 96, Roche LightCycler® 480 II/ Roche cobas z 480 Analyzer and Applied Biosystems 7500.
|Test format||100 reactions|
|Sample preparation||For DNA-preparation the use of SureFast® PREP Bacteria or SureFast® Speed PREP is recommended.|
To assess the process of bacterial growth, it is recommended to compare the samples at the beginning and at the end of the culturing (bacterial growth at Cp difference >3).
|Detection limit||The SureFast® Vibrio 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.|