SureFast® Foodborne Pathogens 4plex
Art. No. F5175
Product info about SureFast® Foodborne Pathogens 4plex
Intended use
The SureFast® Foodborne Pathogens 4plex is a multiplex real-time PCR for for the direct, qualitative detection and differentiation of Escherichia coli virulence factors (stx1 [subtype a-d], stx2 [subtype a-g]), Listeria monocytogenes and Salmonella spp..
Each reaction contains an internal amplification control.
General Information
The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time.
The technical verification of instruments was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Agilent AriaDx and Agilent Mx3005P.
| Specifications | |
|---|---|
| Art. No | F5175 |
| Test format | 100 reactions |
| Sample preparation | For DNA-preparation the use of SureFast® PREP Bacteria is recommended. |
| LOD (Detection Limit) | The SureFast® Foodborne Pathogens 4plex real-time PCR has a limit of detection of < 5 DNA copies. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content. |
