Enzytec™ Sodium
Art. No. E2590
Analytes
Product info about Enzytec™ Sodium
Intended use
Enzytec™ Sodium is an enzymatic assay for the determination of sodium in foodstuff and other sample materials.
General Information
The determination of Sodium by enzymatic way is through the use of a beta-Galactosidase (GAL) sodium-dependent, in the presence of ONPG (o-nitrophenyl-beta-D-galactopyranoside) as substrate. The increase of absorbance makes it possible to assess the concentration of sodium in the sample at 405 nm.
Using the standards contained in the kit, traceable to the NIST 909b Reference Material, it is possible to prepare a 2 point Calibration Curve to refer.
Plotting on the Calibration Curve absorbance values and concentration for each single sample, may be determined the concentration of each sample.
| Specifications | |
|---|---|
| Art. No | E2590 |
| Test format | Test kit for 400 tests on automatic analyzer Reagents: # 1: Buffer (> 0.050 mol/L): 4 bottles of approx. 20 mL # 2: R2 - GAL (GAL < 50 KU/L): 4 bottles of approx. 20 mL # 3: R3 - DIL (Diluent < 0.2 mol/L): 4 bottles of approx. 8.5 mL # 4: R4 - ONPG (ONPG < 50 mmol/L): 2 bottles of approx. 8.5 mL # 5: Liquid Standard 1: 1 vial of approx. 5 mL (Na+ = 2.759 g/L) # 6: Liquid Standard 2: 1 vial of approx. 5 mL (Na+ = 4.138 mg/L) |
| Detected analyte | Sodium |
| Evaluation | Enzymatic assay (405 nm) |