SureFood® ANIMAL ID Pork IAAC

Art. No.: S6114

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SureFood® ANIMAL ID Pork IAAC

Art. No.: S6114

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Intended use

The test detects pork DNA (Sus scrofa). Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC).

 

General Information

The real-time PCR assay can be used with established real-time PCR instruments, equipped for detection of two fluorescence emissions at 522 nm and 553 nm at the same time. The technical validation of instruments was performed on Roche LightCycler® 2.0*, Roche LightCycler® 480 II, Qiagen Rotor-Gene Q, Applied Biossystems 7500, Bio-Rad CFX96 and Agilent Mx3005P.

*note: A Color Compensation is necessary for users of the Roche LightCyclerR 2.0. The SureCC Color Compensation kit II (Art. No. F4010) must be used for the color compensation of such devices.

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Specifications
Art. NoS6114
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Basic and for highly processed food and feed the use of SureFood® PREP Advanced is recommended.
LOD (Detection Limit)The ANIMAL ID Pork IAAC real-time PCR is developed for the detection of pork-DNA in muscle meat mixtures at a relative amount of ≤ 0.5 %. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.