Intended use:

The test detects Bt63 Rice DNA. The detection of Bt63 Rice is a construct-specific real-time PCR assay. Additionally the SureFood® GMO ID Bt63 Rice kit contains a reference-PCR system for the detection of rice.

The real time PCR system used in the SureFood® GMO ID Bt63 rice test is according to the method published by: D. Maede et al.; European Food Research and Technology, 224:271-278 (2006) and therefore according to Commission decision 2008/289/EC.


General Information:

The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).


Article NumbersS2024
Test format 100 reactions
Sample preparation Der real-time PCR Kit ist für eine DNA-Präparation mit dem SureFood® PREP Basic und SureFood® PREP Advanced Kit ausgelegt. Durch die Verwendung von SureFood® PREP Advanced Kit Protokoll 1 (Aufarbeitung von 100 mg der homogenen Probe mit 1,3 mL Lysis Buffer) erhöht sich die DNA-Ausbeute. Damit wird die Nachweisgrenze von 0,01 % bei unbehandelten Reiskörnern gewährleistet.
LOD (Detection limit) The SureFood® GMO ID Bt63 Rice assay has a limit of detection of ≤ 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed rice grain. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
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