Real-time PCR
Moleculaire diagnostiek in de voedingsanalyse
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Technologieën
Real-time PCR
PCR (Polymerase Chain Reaction) is een belangrijke analysemethode in zowel de voedingsmiddelenanalyse alsook de medische diagnostiek.
Een real-time PCR-test dupliceert en analyseert specifieke DNA-sequenties. In de voedingsmiddelenanalyse worden PCR-testen in veel toepassingen gebruikt, bijvoorbeeld voor de detectie van pathogene micro-organismen, voor allergeenidentificatie, voor de detectie van genetisch gemodificeerde organismen (GMO: Genetically Modified Organism) of voor de identificatie van diersoorten. PCR-testen bieden vele voordelen: Ze zijn zeer specifiek, gevoelig, robuust, snel en betrouwbaar. Bovendien kunnen de testen worden geautomatiseerd.
- Veilig: specifieke en gevalideerde methode
- Betrouwbaar: op kwaliteit gecontroleerde testkits
- Gebruiksvriendelijk: gemakkelijk te gebruiken, universele monstervoorbereiding
- Effectief: kan multiplexen en kwantificeren
- Flexibel: veel toepassingsgebieden
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Real-time PCR explained
De eerste stap van elke analyse bestaat uit representatieve bemonstering en homogenisatie. Na lysis van de organische cellen in voedingsweefsel wordt het DNA geëxtraheerd met behulp van verschillende specifieke DNA- of RNA-extractiekits, ofwel voor bacteriën dan wel voor virussen.
SureFood® PREP Basic en SureFood® Prep Advanced kits worden gebruikt voor vaste voeding en diervoeder afkomstig van planten of vlees. Voor dranken worden specifieke DNA-extractiekits gebruikt afhankelijk van de matrix, terwijl de nieuwe QuickGEN lijn een gemakkelijke recht-toe-recht-aan uitvoering mogelijk maakt.
Het modulaire systeem van DNA-extractie en DNA-detectie kan onafhankelijk worden gebruikt. Amplificatie van het doel-DNA vereist naast de relevante kits het gebruik van een geschikte thermocycler met twee (FAM/HEX) tot vier (FAM/HEX/ROX/Cy5) detectiekanalen.
Toepassingsgebieden voor PCR bij het testen van voeding
Voedselallergieën kunnen ernstige immunologische reacties veroorzaken. Om de consumenten te beschermen, moeten voedingsmiddelen worden getest op allergene stoffen. Wij bieden een breed assortiment aan van PCR-kits voor de detectie van allergenen, zoals soja, hazelnoot, pinda, gluten, amandel, selderij, sesam, mosterd, vis en schaaldieren. Wij bieden ook multiplex-testen aan voor de gelijktijdige detectie van meerdere allergenen (pinda/hazelnoot/walnoot en selderij/soja/mosterd).
Specificatie van soorten is van groot belang: met vleesproducten is fraude mogelijke middels onjuiste etikettering (zie het paardenvleesschandaal). Een andere toepassing is de nalevingsgarantie van religieuze voedingsregels (halal, koosjer). Onze SureFood® Animal ID productlijn maakt gevoelig testen voor producten van varken, rund, paard, ezel, kip, kalkoen en andere soorten mogelijk. Met de SureFood® Fish ID productlijn bieden we een breed assortiment aan van PCR-kits voor de identificatie van vissoorten.
Bij de productie van dranken is de detectie van drankbedervers van cruciaal belang. Met de GEN-IAL® productielijn bieden we een breed assortiment aan van screeningkits voor de detectie van bederforganismen in de bier- en wijnproductie, bijvoorbeeld Lactobacillus, Pediococcus, Megasphaera, Pectinatus, Dekkera bruxellensis en Oenococcus oeni. De testkits kunnen worden gebruikt voor zowel controle tijdens het proces, alsook de eindproductcontrole.
De afgelopen jaren is de wereldwijde productie van genetisch gemodificeerde voeding gestaag toegenomen. Omdat voedingsmiddelen die genetisch gemodificeerde organismen (GMO: Genetically Modified Organism) bevatten, moeten worden geëtiketteerd, neemt de vraag naar gevoelige testmethoden toe. Onze SureFood® GMO productlijn maakt de kwalitatieve en kwantitatieve detectie van GMO’s mogelijk – zoals genetisch gemodificeerde mais, LibertyLink-rijst of Roundup Ready-soja.
De detectie van microbiologische besmetting is een van de voornaamste toepassingsgebieden van PCR in de voedingsmiddelenanalyse. Onze productlijn SureFast® detecteert een breed scala aan pathogene bacteriën (bijv. Salmonella, Listeria, Legionella, E. coli, Staphylococcus aureus, Campylobacter), virussen (bijv. Norovirus, Hepatitis en Influenza), gisten en schimmels in voeding.
Productoverzicht voor real-time PCR
U vindt een uitgebreid overzicht van onze SureFood®/SureFast® PCR-kits in onze gratis brochure.
Product portfolio
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS |
The SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA – sequences. Phosphinothricin-Acetyltransferase gene … Read more |
100 reactions | S2128 |
| SureFood® GMO ID 4plex Corn I |
The SureFood® GMO ID 4plex Corn I is a real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified corn DNA sequences: FAM channel: MON810 corn (OECD unique identifier MON-ØØ81Ø-6) VIC/HEX … Read more |
100 reactions | S2170 |
| SureFood® GMO ID 4plex Soya II |
SureFood® GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modified soya DNA sequences listed as below: Roundup Ready Soya (RR Soya) (OECD unique identifier … Read more |
100 reactions | S2162 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| GEN-IAL® QuickGEN Wine Screening white |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must. Read more |
48 reactions | Q323 |
| GEN-IAL® QuickGEN Wine Screening low |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must. Read more |
48 reactions | Q322 |
| GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high |
Detection of Saccharomyces diastaticus in beverages by real-time PCR. Read more |
48 reactions | Q181 |
| GEN-IAL® QuickGEN Yeast Bottom fermented white |
Detection of bottom-fermented yeast in beverages by real-time PCR. Read more |
48 reactions | Q163 |
| GEN-IAL® QuickGEN Yeast Bottom fermented low |
Detection of bottom-fermented yeast in beverages (e.g. beer, wine, grape must) by real-time PCR. Read more |
48 reactions | Q162 |
| GEN-IAL® QuickGEN® Yeast Top fermented white |
Real-time PCR-Kit for the detection of top-fermented yeast. Read more |
48 reactions | Q153 |
| GEN-IAL® QuickGEN® Yeast Top fermented low |
Real-time PCR-Kit for the detection of top-fermented yeast. Read more |
48 reactions | Q152 |
| GEN-IAL® QuickGEN Beer differentiation white |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. … Read more |
12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. | Q083 |
| GEN-IAL® QuickGEN Beer differentiation low |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeast are differentiated: L. acetotolerans, L. backii, L. brevis/L. brevisimilis/L. parabrevis, L. lindneri, L. casei/ L paracasei, L. … Read more |
12 strips à 8 tubes (one strip per sample, tube 2 contains lyticase) for 12 samples. | Q082 |
| GEN-IAL® QuickGEN Beer yeast and bacteria differentiation white |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild … Read more |
96 PCR reactions (24 samples) 12 stripes (tubes 2, 3, 5 and 6 contain lyticase) |
Q073 |
| GEN-IAL® QuickGEN Beer yeast and bacteria differentiation low |
Differentiation of beer spoilage bacteria and yeast in beer and mixed beer drinks. The following bacteria and yeasts are differentiated: Lactobacilli Pediococci Acetic acid bacteria Enterobacteriaceae Bottom-fermented yeast Top-fermented yeast Wild … Read more |
96 PCR reactions (24 samples) 12 stripes |
Q072 |
| GEN-IAL® QuickGEN P1 Screening and Hop resistance |
Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Read more |
50 reactions | Q055 |
| GEN-IAL® QuickGEN P1 Screening and Hop resistance low MG |
Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Read more |
48 reactions | Q054 |
| GEN-IAL® QuickGEN P1 Screening and Hop resistance white |
Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Read more |
48 reactions | Q053 |
| GEN-IAL® QuickGEN P1 Screening and Hop resistance low |
Qualitative detection of bacterial contaminations and the hop resistance genes hor A and hor C as genetic markers for beer-spoilage microorganisms in beer and mixed beer drinks. Read more |
48 reactions | Q052 |
| GEN-IAL® QuickGEN P1 and S. diastaticus Screening |
Qualitative detection of bacterial and S. cerevisiae var. diastaticus contaminations in filtrated beer, beer mixes and yeast containing samples. Read more |
50 reactions | Q045 |
| GEN-IAL® QuickGEN P1 and S. diastaticus Screening low MG |
Qualitative detection of bacterial and S.diastaticus contaminations in filtrated beer and mixed beer drinks and yeast containing samples. Read more |
48 reactions | Q044 |
| GEN-IAL® QuickGEN P1 and S. diastaticus Screening white |
Qualitative detection of bacterial and S.diastaticus contaminations in filtrated beer and mixed beer drinks. Read more |
48 reactions | Q043 |
| GEN-IAL® QuickGEN P1 and S. diastaticus Screening low |
Qualitative detection of bacterial and S. cerevisiae var. diastaticus contaminations in filtrated beer, beer mixes and yeast containing samples. Read more |
48 reactions | Q042 |
| GEN-IAL® QuickGEN P1 Screening without yeast |
Qualitative detection of bacterial contaminations in beer and mixed beer drinks. Read more |
50 reactions | Q035 |
| GEN-IAL® QuickGEN P1 Screening without yeast white |
Qualitative detection of bacterial contaminations in beer and mixed beer drinks. Read more |
48 reactions | Q033 |
| GEN-IAL® QuickGEN P1 Screening without yeast low |
Qualitative detection of bacterial contaminations in beer and mixed beer drinks. Read more |
48 reactions | Q032 |
| GEN-IAL® QuickGEN P1 Screening |
Qualitative detection of bacterial and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find bacterial contaminations more sensitive (Q035). Read more |
50 reactions | Q025 |
| GEN-IAL® QuickGEN P1 Screening low MG |
Qualitative detection of bacterial and yeast contaminations in filtrated beer and beer mixes. Read more |
48 reactions | Q024 |
| GEN-IAL® QuickGEN P1 Screening white |
Qualitative detection of bacterial and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find bacterial contaminations more sensitive (Q033). Read more |
48 reactions | Q023 |
| GEN-IAL® QuickGEN P1 Screening low |
Qualitative detection of bacterial and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find bacterial contaminations more sensitive (Q032). Read more |
48 reactions | Q022 |
| SureFast® Escherichia coli Serotype II 4plex |
The SureFast® Escherichia coli Serotype II 4plex is a real-time PCR for the direct, qualitative detection and differentiation of specific wzx gene DNA sequences of Escherichia coli O45, wbdl gene DNA sequences of Escherichia coli O111 and ihp1 gene … Read more |
100 reactions | F5168 |
| SureFast® Escherichia coli Serotype I 4plex |
The SureFast® Escherichia coli Serotype I 4plex is a real-time PCR kit for the direct, qualitative detection and differentiation of specfifc wzx gene DNA sequences of Escherichia coli O121, O26 and O103. Read more |
100 reactions | F5167 |
| GEN-IAL® QuickGEN Wine Screening without yeast high |
Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must. Read more |
48 reactions | Q331 |
| GEN-IAL® QuickGEN Wine Screening high |
Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must. Read more |
48 reactions | Q321 |
| SureFast® Foodborne Pathogens 4plex |
The SureFast® Foodborne Pathogens 4plex is a real-time PCR for for the direct, qualitative detection and differentiation of Escherichia coli virulence factors (stx1 [subtype a-d], stx2 [subtype a-g]), Listeria monocytogenes and Salmonella spp.. Each … Read more |
100 reactions | F5175 |
| GEN-IAL® QuickGEN Wild yeast 2 |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia … Read more |
50 reactions | Q535 |
| GEN-IAL® QuickGEN Wild yeast 1 low |
Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila … Read more |
48 reactions | Q522 |
| SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex |
The SureFast® Salmonella Species/Enteritidis/Typhimurium 4plex is a real-time PCR for the direct, qualitative detection and differentiation of Salmonella spp., Salmonella Enteritidis and Salmonella Typhimurium. Each reaction contains an internal … Read more |
100 reactions | F5166 |
| GEN-IAL® QuickGEN P1 Screening high |
Qualitative detection of bacterial and yeast contaminations in filtrated beer and beer mixes. For products with high amount of yeast use the Screening Kit without yeast detection to find bacterial contaminations more sensitive (Q031). Read more |
48 reactions | Q021 |
| GEN-IAL® QuickGEN Enterobacteriacea spp. |
Qualitative multiplex PCR-detection of enterobacteriaceae as indicators for hygiene and postprocessing contaminations. The system contains an integrated inhibition control. Read more |
50 reactions | Q145 |
| GEN-IAL® QuickGEN Lactobacillus spp. / Pediococcus spp. |
Qualitative detection of Lactobacillus spp. and Pediococcus spp. in beverages. Read more |
48 reactions tube strips (freeze-dried, incl. IC-DNA) |
QTLP0048* |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| SureFood® ALLERGEN 4plex Cereals |
The SureFood® ALLERGEN 4plex Cereals is a real-time PCR test for the direct, qualitative detection and differentiation of specific wheat (Triticum), barley (Hordeum vulgare) and rye (Secale cereale) DNA sequences in food. Each reaction contains an … Read more |
100 reactions | S7006 |
| SureFood® Apricot |
The real-time PCR test detects DNA of apricot (Prunus armenica). Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S7007 |
| SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC |
The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya … Read more |
100 reactions | S3403 |
| Product | Description | No. of tests/amount | Art. No. |
|---|---|---|---|
| SureFood® ANIMAL QUANT Turkey |
The SureFood® ANIMAL QUANT Turkey real-time PCR detects the relative turkey (Meleagris gallopavo) DNA amount to the total animal (amnions) DNA content in meat samples. Therefore the kit contains two PCR systems, one for detection of a turkey … Read more |
2 x 50 reactions | S1015 |
| SureFood® Apricot |
The real-time PCR test detects DNA of apricot (Prunus armenica). Each reaction contains an internal amplification control (IAC). Read more |
100 reactions | S7007 |
| SureFood® ANIMAL ID 4plex Camel/Horse/Donkey + IAAC |
The test detects camel DNA of Arabian camel (Camelus dromedarius) and of Bactrian camel (Camelus bactrianus), horse DNA (Equus caballus) and donkey DNA (Equus asinus). Each reaction contains an internal amplification control and an internal detection … Read more |
100 reactions | S6131 |
| SureFood® ANIMAL ID 3plex Water Buffalo/Beef+IAAC |
The test detects water buffalo (Bubalis arnee) and beef (Bos taurus) DNA. Each reaction contains an internal amplification control and an internal detection assay for vertebrates DNA (IAAC). Read more |
100 reactions | S6130 |
