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Intended use

EuroProxima Tetracycline is a competitive enzyme immunoassay for quantitative analysis of tetracyclines in tissue/liver, milk, egg, honey, shrimps and butter.

General Information

Tetracylines are a group of antibiotics derived from Streptomyces spp. with a broad spectrum activity against Gram-negative and Gram-positive aerobic and anaerobic bacteria. Because of their broad spectrum activity, low toxicity profile and low cost, tetracyclines are often used as feed additives for food-producing animals (including honeybees) and in aquaculture. The most commonly used tetracyclines in veterinary medicine are tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC). Tetracyclines bind to the 30S subunit of microbial ribosomes. They inhibit protein synthesis by blocking the attachment of aminoacyl-tRNA to the A site on the ribosome. In this way, introduction of new amino acids to the nascent peptide chain is prevented. By inhibiting protein synthesis TCs cause cell death of the bacterial cell. The action of tetracyclines is reversible upon withdrawal of the drug. Residues in food of animal origin may be found, often because of improper observance of withdrawal times. The EU has set maximum residue limits (MRLs) for TC, OTC, CTC and DC: 100 µg/kg in muscle and in milk, 200 µg/kg in egg, 300 µg/kg in liver and 600 µg/kg in kidney. For TC, OTC and CTC these MRLs are expressed as the sum of the parent drug and its 4-epimer, whereas for DC only the parent compound is included in the MRL.