Mycotoxins

Mycotoxin analysis – rapid and reliable

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Analytes

Mycotoxins

Mycotoxins are toxic metabolites produced by molds. They can have serious acute and chronic effects on the health of humans as well as animals. Mycotoxin analysis of food and feed is therefore necessary and often required by legislation. Modern test kits allow rapid qualitative and quantitative analysis of mycotoxins.

Throughout history, mycotoxins (as we know them now) have impaired the quality of agricultural products. Major commodities such as corn, wheat, rice, soy and peanuts can be contaminated with mycotoxins, depending on growth conditions and storage conditions. So can other commodities like rye, oats, hazelnuts, figs, grapes or spices be affected by mycotoxins. The consumption of mycotoxin contaminated products may have acute toxic health effects on humans and animals as well as chronic effects. Besides health costs also direct economic factors play a role like decreased efficiency in the growth of farm animals, trade losses because of import and export limitations and direct losses in revenue of crop value. To protect the health of consumers and animals worldwide, legislations for mycotoxins are in place.

We offer test kits for all relevant mycotoxins from aflatoxin to zearalenone. Please choose a specific mycotoxin to find out more about it:

Methods for mycotoxin analysis

The analysis of mycotoxins presents some unique challenges: Mould fungus toxins are naturally occurring contaminants. Production prior to harvest is affected by weather conditions, as well as agricultural practices; production after harvest depends upon storage conditions. This puts certain challenges on the risk management of mycotoxins: Where, when, how often and how many do I test?

Mycotoxins are spread very inhomogeneously throughout a product and maybe present at very low levels. Samples to be tested may be analytically simple matrices such as wheat, corn or maybe very complicated matrices like spices, coffee or complex mixtures of ingredients as in animal feed. It is very important to choose the right sampling and analytical method for the product to be tested. R-Biopharm offers a wide range of analytical methods for mycotoxin analysis. Depending on matrix, number of samples, time to result, ease of use and available equipment, we can offer you the appropriate testing format:

ELISA (enzyme-linked immunosorbent assays) test kits are accurate and easy to use. ELISA test kits arethe ideal solution for a measurement of multiple samples. R-Biopharm offers two product lines for ELISA:

RIDASCREEN®FAST
Short incubation times of as low as 8 – 15 minutes, for mostly up to 19 samples in single determination in one run.

RIDASCREEN®
Low limit of detection (LOD). With incubation times of e.g. 75 minutes for up to 42 samples in double determination. Due to the longer incubation times these test kits can be automated using the e.g. ThunderBolt®.

The main advantages of ELISA are that it is fast for a high number of samples, low cost for set up and minimal training required. ELISA can be automated using the e.g. ThunderBolt®. Cost effectiveness for automation requires a minimum of 4 to 5 samples tested per ELISA run. Results are measured with an ELISA plate reader, such as the RIDA® Absorbance 96.

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In general, testing of raw ingredients can be done with on-site rapid test. Results are available in minutes and require only a minimal amount of training and equipment. While conventional LFD kits use an organic solvent, more environmentallyfriendly kits use a water extraction to avoid organic solvent waste.

To remove subjectivity in the evaluation, digital reading of quantitative results is carried out with the RIDA®SMART APP.

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The screening cards AFLACARD® and OCHRACARD® are specially designed to get a rapid, semi-quantitative result for more complex commodities.

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Immunoaffinity columns (IAC) (e.g. RIDA®, EASI-EXTRACT®, PREP®) contain monoclonal antibodies making them highly selective. Further advantages are:

  • Excellent sample clean-up, especially with complex commodities. This makes it possible to use solvent based standards with LC-MS/MS detection.
  • Clean eluates mean that there is no requirement for matrix matched or isotopic labelled.

Solid phase extraction columns (SPE) use selected solid adsorbents to bind interfering components and pigments, allowing the mycotoxins to be easily analyzed. Further benefits are:

  • Non-specific clean-up, making this an ideal clean-up tool for the analysis of multi-toxins in simple matrices like cereals.
  • Simple, fast and cost-effective clean-up providing an excellent screening test in conjunction with LC-MS/MS.
  • Can be used in combination with matrix matched standards removing the requirement for expensive isotopic labelled standards.

HPLC and LC-MS/MS are also reference methods if it comes to legal issues. The main advantages of High Performance Liquid Chromatography (HPLC) and LC-MS/MS are their high sensitivity, applicability to complex matrixes and accuracy. However, to obtain top-notch HPLC and LC-MS/MS performance, an optimized sample clean-up using solid phase or immunoaffinity columns are required.

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For very high sample throughput, fully automated systems like CHRONECT Symbiosis RIDA®CREST using immunoaffinity cartridges can be used up to 15 times. With LC-MS/MS all regulated mycotoxins are detected as well as most common masked andemerging metabolites. It is also possible to analyse other parameters such as pesticides and veterinary drugs.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
AFLACARD TOTAL

A qualitative screening card for the detection of different aflatoxin total levels in food and feed samples.

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10 cards containing 20 tests and controls RBRP38
AFLACARD B1

A qualitative screening card for detection of aflatoxin B1 at various levels in food and feed commodities.

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10 cards containing 20 tests and controls RBRP27
OCHRACARD

A qualitative screening card for detection of ochratoxin A at various levels in food and feed commodities.

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10 cards containing 20 tests and controls RBRP48
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ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® Ochratoxin A 30/15

RIDASCREEN® Ochratoxin A 30/15 is a competitive enzyme immunoassay for the quantitative analysis of ochratoxin A in corn, wheat, barley, rye, rice and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R1312
RIDASCREEN® T-2 / HT-2 Toxin

RIDASCREEN® T-2 / HT-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of the sum of T-2 and HT-2 toxin in oats, corn (maize), barley and wheat.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3805
RIDASCREEN® Zearalenon

RIDASCREEN® Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone residues in cereals, feed, beer, serum and urine.

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Microtiter plate with 96 wells (12 strips with 8 wells each). R1401
RIDASCREEN® Fumonisin

RIDASCREEN® Fumonisin is a competitive enzyme immunoassay for the quantitative determination of fumonisin in corn and corn products (corn flour, corn, semolina, polenta, corn for popcorn).

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3401
RIDASCREEN® DON

RIDASCREEN® DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt, feed, beer and wort.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R5906
RIDASCREEN® T-2 Toxin

RIDASCREEN® T-2 Toxin is a competitive enzyme immunoassay for the quantitative determination of T-2 toxin in cereals and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R3801
RIDASCREEN®FAST Zearalenon SC

RIDASCREEN®FAST Zearalenon SC is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5505
RIDASCREEN®FAST Zearalenon

RIDASCREEN®FAST Zearalenon is a competitive enzyme immunoassay for the quantitative analysis of zearalenone in cereals and feed. The test was validated with different matrices of cereals and composed feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5502
RIDASCREEN®FAST T-2 Toxin

RIDASCREEN®FAST T-2 Toxin is a competitive enzyme immunoassay for the quantitative analysis of T-2 toxin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5302
RIDASCREEN®FAST Ochratoxin A

RIDASCREEN®FAST Ochratoxin A is a competitive enzyme immunoassay for the quantitative analysis of ochratoxin A in corn, wheat, barley, oats (grains) and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5402
RIDASCREEN®FAST Fumonisin

RIDASCREEN®FAST Fumonisin is a competitive enzyme immunoassay for the quantitative determination of fumonisin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 removable wells each) R5602
RIDASCREEN®FAST DON SC

RIDASCREEN®FAST DON SC is a competitive enzyme immunoassay for the quantitative determination of DON in cereals, malt and feed with a sinlge control standard curve.

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Microtiter plate with 48 wells (6 strips with 8 wells each). R5905
RIDASCREEN®FAST DON

RIDASCREEN®FAST DON is a competitive enzyme immunoassay for the quantitative analysis of DON in cereals, malt and feed.

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R5901: microtiter plate with 96 wells (12 strips with 8 removable wells each)
R5902: microtiter plate with 48 wells (6 strips with 8 removable wells each)
R5901 / R5902
RIDASCREEN®FAST Citrinin

RIDASCREEN®FAST Citrinin assay is a competitive enzyme immunoassay for the quantitative determination of citrinin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R6302
RIDASCREEN®FAST Aflatoxin SC

RIDASCREEN®FAST Aflatoxin SC is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed with a single control standard curve.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R9002
RIDASCREEN®FAST Aflatoxin M1

RIDASCREEN®FAST Aflatoxin M1 is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin M1 in milk and milk powder.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5812
RIDASCREEN®FAST Aflatoxin

RIDASCREEN®FAST Aflatoxin is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin in cereals and feed.

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Microtiter plate with 48 wells (6 strips with 8 wells each) R5202
RIDASCREEN® Aflatoxin Total

RIDASCREEN® Aflatoxin Total is a competitive enzyme immunoassay for the quantitative determination of aflatoxins in cereals and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R4701
RIDASCREEN® Aflatoxin B1 30/15

RIDASCREEN® Aflatoxin B1 30/15 is a competitive enzyme immunoassay for the quantitative determination of aflatoxin B1 in cereals and feed.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1211
RIDASCREEN® Aflatoxin M1

RIDASCREEN® Aflatoxin M1 is a competitive enzyme immunoassay for the quantitative analysis of aflatoxin M1 in milk and milk powder.

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Microtiter plate with 96 wells (12 strips with 8 wells each) R1121
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ProductDescriptionNo. of tests/amountArt. No.
RIDA®SMART APP

The RIDA®SMART APP is a software application for the quantification of RIDA®QUICK mycotoxin lateral flow tests. The RIDA®SMART APP is a complete new evaluation technology for the quantification of mycotoxins. This technology evaluates lateral flow …

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Android 7.1- Android 11.0 OS based application for

Motorola Nexus 6
Huawei Nexus 6P
Google Pixel
Google Pixel XL
Google Pixel 2
Motorola Moto G6
Xiaomi RedMi Note 7
Xiaomi RedMi Note 9S
Xiaomi RedMi Note 10S
ZRSAM1000
RIDA®QUICK Aflatoxin RQS ECO

RIDA®QUICK Aflatoxin RQS ECO is a quantitative immunochromatographic test in a test strip format to determine aflatoxin (sum B1, B2, G1, G2) in corn. The test uses an aqueous extraction method. Results are evaluated with the RIDA®SMART APP software …

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20 x test strips R5209
RIDA®QUICK Aflatoxin RQS

RIDA®QUICK Aflatoxin RQS is a quantitative immunochromatographic test in strip format for the determination of aflatoxin in corn. Results are evaluated with the RIDA®SMART APP software (Art. No. ZRSAM1000) or the RIDA®QUICK SCAN reader.

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20 x test strips R5208
RIDA®QUICK DON RQS ECO

RIDA®QUICK DON RQS ECO is a quantitative immunochromatographic test with water extraction in a test strip format to determine DON in grains (wheat, corn, barley, oats). Results are evaluated with the RIDA®SMART APP software (Art. No. ZRSAM1000) or …

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20 x test strips R5911
RIDA®SMART APP

The RIDA®SMART APP is a software application for the quantification of RIDA®QUICK mycotoxin lateral flow tests. The RIDA®SMART APP is a complete new evaluation technology for the quantification of mycotoxins. This technology evaluates lateral flow …

Read more
Android 7.1- Android 11.0 OS based application for

Motorola Nexus 6
Huawei Nexus 6P
Google Pixel
Google Pixel XL
Google Pixel 2
Motorola Moto G6
Xiaomi RedMi Note 7
Xiaomi RedMi Note 9S
Xiaomi RedMi Note 10S
ZRSAM1000
RIDA®QUICK T-2 / HT-2 RQS ECO

RIDA®QUICK T-2 / HT-2 RQS ECO is a immunochromatographic test in strip format for the quantitative determination of the sum of T-2 / HT-2 toxin in oats, wheat and corn (maize). Results are evaluated with the RIDA®SMART APP software (Art. No. …

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20 x test strips R5304
RIDA®QUICK Zearalenon RQS

RIDA®QUICK Zearalenon RQS is a quantitative immunochromatographic test in strip format for the detemination of zearalenone in corn. Results are evaluated with the RIDA®SMART APP software (Art. No. ZRSAM1000) or the RIDA®QUICK SCAN reader.

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20 x test strips R5504
RIDA®QUICK Fumonisin RQS ECO

RIDA®QUICK Fumonisin RQS ECO is a quantitative immunochromatographic strip test with an aqueous extraction method for the detection of fumonisin in corn. Results are evaluated with the RIDA®SMART APP software (Art. No. ZRSAM1000) or the RIDA®QUICK …

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20 x test strips R5606
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ProductDescriptionNo. of tests/amountArt. No.
IMMUNOPREP® ONLINE DEOXYNIVALENOL

Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of deoxynivalenol prior to HPLC or LC-MS/MS.

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RBRP902/48: 48 cartridges RBRP902/48
IMMUNOPREP® ONLINE OCHRATOXIN

Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of ochratoxin A prior to HPLC or LC-MS/MS.

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RBRP901/48: 48 cartridges,
RBRP901: 96 cartridges
RBRP901 / RBRP901/48
IMMUNOPREP® ONLINE AFLATOXIN M1

Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of aflatoxins M1 prior to HPLC or LC-MS/MS.

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RBRP904/48: 48 cartridges RBRP904/48
IMMUNOPREP® ONLINE AFLATOXIN

Online immunoaffinity cartridges used in conjunction with the RIDA®CREST handling system for the automated clean-up and analysis of aflatoxins B1, B2, G1 and G2 prior to HPLC or LC-MS/MS.

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RBRP900/48: 48 cartridges,
RBRP900: 96 cartridges
RBRP900 / RBRP900/48
11+Myco MS-PREP®

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, Deoxynivalenol, Zearalenone, T-2 and HT-2 in a wide range of commodities in conjunction with LC-MS/MS.

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RBRP128: 10 immunoaffinity columns
(3 ml format),
RBRP128B: 50 immunoaffinity columns
(3 ml format)
RBRP128 / RBRP128B
EASI-EXTRACT® CITRININ

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of citrinin in a wide range of commodities.

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10 columns (3 ml format) (RBRDP126),
25 columns (3 ml format) (RBRP126)
RBRDP126 / RBRP126
PuriTox Total Myco-MS

Solid phase clean-up columns for the purification of 11 mycotoxins. PuriTox Total Myco-MS® (Art. No. TC-MT3000) can be used for the simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A, fumonisins B1 and B2, deoxynivalenol, zearalenone, …

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25 solid phase clean-up columns (syringe format) TC-MT3000
AFLAPREP® M WIDE

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxin M1 in milk and …

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10 columns (3 ml format) (RBRP124),
50 columns (3 ml format) (RBRP124B)
RBRP124 / RBRP124B
AOF MS-PREP®

AOF MS-PREP® immunoaffinity columns contain highly specific monoclonal antibodies directed against aflatoxin, ochratoxin A and fumonisin. The columns lead not only to a clean-up of the sample but also to a selective purification of the mycotoxins. …

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RBRP115: 10 immunoaffinity columns
(3 ml format),
RBRP115B: 50 immunoaffinity columns
(3 ml format)
RBRP115 / RBRP115B
OCHRAPREP®

Immunoaffinity columns for use in conjunction with HPLC or LC-MS/MS for detection of ochratoxin A in a wide range of commodities.

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10 columns (3 ml format) (RBRP14),
50 columns (3 ml format) (RBRP14B)
RBRP14 / RBRP14B
EASI-EXTRACT® ZEARALENONE

Immunoaffinity columns for use in conjunction with HPLC or LC-MS/MS for detection of zearalenone in a wide range of commodities.

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10 columns (3 ml format) (RBRRP91),
50 columns (3 ml format) (RBRRP90)
RBRRP91 / RBRRP90
EASI-EXTRACT® T-2 & HT-2

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of T-2 and HT-2 toxins in a wide range of commodities.

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10 columns (3 ml format) (RBRP43),
50 columns (3 ml format) (RBRP43B)
RBRP43 / RBRP43B
EASIMIP™ PATULIN

Molecularly Imprinted Polymer (MIP) columns for use in conjunction with an HPLC for detection of patulin in apple juice and apple purée.

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RBRP250: 10 columns (3 ml format),
RBRP250B: 50 columns (3 ml format)
RBRP250 / RBRP250B
RIDA® Ochratoxin A column

RIDA® Ochratoxin A column are immunoaffinity columns for sample clean up prior to analysis of Ochratoxin A in food and feed.

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10 immunoaffinity columns R1303
AFLAOCHRA PREP®

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2 and ochratoxin A in a wide range of commodities in conjunction with HPLC or LC-MS/MS.

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RBRP89: 10 immunoaffinity columns
(1 ml format),
RBRP89B: 50 immunoaffinity columns
(1 ml format)
RBRP89 / RBRP89B
DZT MS-PREP®

DZT MS-PREP® immunoaffinity columns contain highly specific monoclonal antibodies directed against DON, zearalenone, T-2 and HT-2 toxin. The columns lead not only to a clean-up of the sample but also to a selective purification of the mycotoxins. …

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RBRP73: 10 immunoaffinity columns
(1 ml format),
RBRP73B: 50 immunoaffinity columns
(1 ml format)
RBRP73 / RBRP73B
AO ZON PREP®

Multi-mycotoxin immunoaffinity columns for simultaneous detection of aflatoxins B1, B2, G1, G2, ochratoxin A and zearalenone in a wide range of commodities in conjunction with HPLC or LC-MS/MS.

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RBRP112: 10 immunoaffinity columns
(3 ml format),
RBRP112B: 50 immunoaffinity columns
(3 ml format)
RBRP112 / RBRP112B
FUMONIPREP®

Immunoaffinity columns are used in conjunction with HPLC or LC-MS/MS for detection of fumonisins B1 and B2 in a wide range of commodities.

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10 columns (3 ml format) (RBRDP31),
50 columns (3 ml format) (RBRP31B)
RBRDP31 / RBRP31B
DONPREP®

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of deoxynivalenol in a wide range of commodities.

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10 columns (3 ml format) (RBRP50),
50 columns (3 ml format) (RBRP50B)
RBRP50 / RBRP50B
EASI-EXTRACT® AFLATOXIN

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS for detection of aflatoxins B1, B2, G1, G2, M1 and M2 in a wide range of commodities.

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10 columns (3 ml format) (RBRRP71),
50 columns (3 ml format) (RBRRP70N)
RBRRP71 / RBRRP70N
RIDA® Aflatoxin column

RIDA® Aflatoxin columns (Art. No. R5001/R5002) are immunoaffinity columns for sample clean up prior to analysis of aflatoxins B1, B2, G1, G2 in food and feed. The columns are particularly suited for the clean up of difficult sampIes such as nuts, …

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10 immunoaffinity columns (1 ml format) (R5001),
50 immunoaffinity columns (1 ml format) (R5002)
R5001 / R5002
PuriTox Aflatoxin

Solid phase clean-up columns for the purification of aflatoxins B1, B2, G1 and G2 from peanuts.

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50 columns (syringe format) RBRP25
AFLAPREP®

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform.

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10 columns (1 ml format) (RBRDP07),
50 columns (1 ml format) (RBRP07)
RBRDP07 / RBRP07
AFLAPREP® M

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform.

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10 columns (1 ml format) (RBRDP04) ,
25 columns (1 ml format) (RBRP04)
RBRDP04 / RBRP04
PuriTox Trichothecene

Solid phase clean-up columns for the purification of cereal samples to detect trichothecenes. PuriTox Trichothecene can be used for the simultaneous detection of type A and B trichothecenes by GC and LC-MS/MS.

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25 solid phase clean-up columns (syringe format) TC-T220
PuriTox AflaZON

Solid phase clean-up columns for the purification of aflatoxins and zearalenone from cereals.

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25 solid phase clean-up columns (syringe format) TC-M160
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