Wine analysis

Detection of spoilage organisms in wine

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Wine analysis

Do you want to test wine for beverage spoilers? For fast and specific screening and detection of microbiological spoilage parameters in wine, a wide product line of qPCR kits is offered.

We offer several qualitative real-time PCR test kits for wine analysis, e.g. for detection of the yeast Brettanomyces (Dekkera bruxellensis) which may spoil the taste and flavour of the wine. The following spoilage bacteria and yeasts are of special interest in the wine industry and should be monitored:

Dekkera bruxellensis (anamorph Brettanomyces bruxellensis) is a spoilage yeast in beverages, especially important for the wine industry, where it has been shown to produce high amounts of volatile phenols (4-ethylphenol and 4-ethylguaiacol) imparting off-flavors to red wines. The yeasts of the species Dekkera bruxellensis are responsible for serious economic losses in the wine industry owing to their ability to spoil wines by the production of ethylphenols. Some authorities consider “brett” to be responsible for 90% of the spoilage problems in premium red wines. However, in the production of beer (especially craft beer or Belgian beer specialities), Dekkera bruxellensis is often not considered as contaminant, but as flavor enrichment.

In wine production, the malolactic fermentation is supported by Lactobacillus. During this process, the bacterium Oenococcus oeni can be added as highly specialized bacteria. It effects a transformation of malic acid into milder lactic acid. However, depending on the desired type of wine, the presence of Oenococcus oeni may also be undesirable.

Pediococcus is a genus of gram-positive lactic acid bacteria which are considered as contaminant in wine and beer. However, in some beer styles, the presence of Pediococcus may be desired.

Pichia is a genus of yeasts in the family Saccharomycetaceae. It is used in the fermentation process in winemaking, but it can spoil wine when present in high quantities. Pichia may also cause turbidity and off-flavors in beer.

Saccharomyces is a genus of fungi including various species of yeasts. One of the best known species is Saccharomyces cerevisiae which is used in the production of beer, wine or bread. The species Saccharomyces diastaticus is a spoilage yeast in the wine and beer industry.

Molecularbiological wine analysis

You can find more information on real-time PCR wine analysis in our free brochure.

Test kits for wine analysis

Our GEN-IAL® portfolio includes multiplex screening kits and wine-specific kits. The new GEN-IAL® QuickGEN line is characterized by a simple and rapid sampling and assay process, providing results within two hours after sampling.

ProductDescriptionTubeArt. No.
GEN-IAL® QuickGEN Wine ScreeningQualitative detection and differentiation of bacterial and yeast contaminations in wine and grape musthighQ321
lowQ322
whiteQ323
MGQ324
ProductDescriptionTubeArt. No.
GEN-IAL® QuickGEN Wine Screening without yeastQualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteriahighQ331
lowQ332
whiteQ333
MGQ334
GEN-IAL® Biogenic AminesSpecific DNA detection of bacteria forming biogenic aminesQ345
GEN-IAL® QuickGEN Oenococcus OeniSpecific DNA detection of Oenococcus oenihighQ351
lowQ352
whiteQ353
Q355
GEN-IAL® QuickGEN Acetic acid bacteriaSpecific DNA detection of acetic acid bacteriahighQ511
lowQ512
whiteQ513
Q515
ProductDescriptionTubeArt. No.
GEN-IAL® QuickGEN Yeast Dekkera bruxellensis quantitativeSpecific DNA detection of Dekkera bruxellensisSPQ370
highQ371
lowQ372
whiteQ373
GEN-IAL® Dekkera bruxellensis quantitativeSpecific DNA detection of Dekkera bruxellensisSPQ380
Specific DNA detection of Dekkera bruxellensis (FAM/ROX)Q385
Specific DNA detection of Dekkera bruxellensis (FAM/HEX)Q395
GEN-IAL® QuickGEN Yeast Dekkera spp.Specific DNA detection of Dekkera spp.highQ551
lowQ552
whiteQ553
Q555
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailiiSpecific DNA detection of Zygosaccharomyces bailiihighQ561
lowQ562
whiteQ563

DNA preparation:

ProductDescriptionArt. No.
GEN-IAL® Simplex® Easy WineDNA preparation of wine samplesQ300
GEN-IAL® QuickGEN Sample Preparation CentrifugationDNA preparation of beverage samples, centrifugationQ002

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
QuickGEN PCR Kit Yeast universal -low-

Qualitative detection of yeast contaminations in beverages. For exapmple: Saccharomyces spp. Dekkera spp. Zygosaccharomyces spp. Rhodotorula spp. Torulaspora spp. Kluyveromyces spp. Debaromyces spp. Candida spp. Hanseniaspora spp. Pichia spp

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48 reactions: 48 x Dye Strips (freeze-dried, incl. IC-DNA) Q982
GEN-IAL® Dekkera bruxellensis Standard DNA

Quantification of Dekkera bruxellensis in beverages (e.g. wine and grape must) by real-time PCR.

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1 x Standard 1 200000 cfus / 2.5 μL DNA (D. bruxellensis, freeze-dried) Q360
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii white

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions Q563
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii low

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

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48 reactions (tubes incl. Lyticase and IC-DNA) Q562
GEN-IAL® QuickGEN Yeast Dekkera spp.

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus.

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50 reactions Q555
GEN-IAL® QuickGEN Yeast Dekkera spp. white

Detection of Dekkera spp. in wine and grape must by real-time PCR.

Read more
48 reactions Q553
GEN-IAL® QuickGEN Yeast Dekkera spp. low

Detection of Dekkera spp. in beverages (e.g. beer, wine, grape must) by real-time PCR. The following species are detected: D. bruxellensis, D. anomala, D. custersiana, D. naardenensis and D. nanus

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48 reactions Q552
GEN-IAL® QuickGEN Yeast Dekkera spp. high

Detection of Dekkera spp. in wine and grape must by real-time PCR.

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48 reactions Q551
GEN-IAL® QuickGEN Yeast differentiation white

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples Q543
GEN-IAL® QuickGEN Yeast differentiation low

Differentiation of 12 beer spoilage yeasts in beer and mixed beer drinks: Saccharomyces exiguus Saccharomyces cerevisiae var. diastaticus Saccharomyces bayanus / pastorianus Saccharomycodes ludwigii Torulaspora delbrückii Klyveromyces marxianus …

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96 reactions for 12 samples (tubes contain lyticase). Q542
GEN-IAL® QuickGEN Wild yeast 2 low

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia …

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48 reactions Q532
GEN-IAL® QuickGEN Wild yeast 1

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila …

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50 reactions Q525
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/HEX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

Read more
50 reactions Q395
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative FAM/ROX

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

Read more
50 reactions Q385
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative white

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

Read more
48 reactions Q373
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative low

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

Read more
48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). Q372
GEN-IAL® QuickGEN Wine Screening without yeast low MG

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

Read more
48 reactions Q334
GEN-IAL® QuickGEN Wine Screening without yeast white

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

Read more
48 reactions Q333
GEN-IAL® QuickGEN Wine Screening without yeast low

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

Read more
48 reactions Q332
GEN-IAL® QuickGEN Wine Screening low MG

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

Read more
48 reactions Q324
GEN-IAL® QuickGEN Wine Screening white

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

Read more
48 reactions Q323
GEN-IAL® QuickGEN Wine Screening low

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

Read more
48 reactions Q322
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus high

Detection of Saccharomyces diastaticus in beverages by real-time PCR.

Read more
48 reactions Q181
GEN-IAL® QuickGEN Wine Screening without yeast high

Qualitative detection of Lactobacilli, Pediococci, Oenococcus oeni and acetic acid bacteria in wine and grape must.

Read more
48 reactions Q331
GEN-IAL® QuickGEN Wine Screening high

Qualitative detection and differentiation of bacterial and yeast contaminations in wine and grape must.

Read more
48 reactions Q321
GEN-IAL® QuickGEN Wild yeast 2

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Candida glabrata Candida albicans Candida kefyr Candida intermedia Candida parapsilosis Candida sake Candida tropicalis Naumovozyma dairenensis Pichia …

Read more
50 reactions Q535
GEN-IAL® QuickGEN Wild yeast 1 low

Detection of wild yeast in beverages by real-time PCR. The following yeasts are detected: Dekkera anomala Dekkera bruxellensis Dekkera custersiana Dekkera naardenensis Debaromyces hansenii Hanseniaspora guillermondii Hanseniaspora osmophila …

Read more
48 reactions Q522
GEN-IAL® QuickGEN Yeast Saccharomyces diastaticus low

Detection of Saccharomyces diastaticus in beverages by real-time PCR.

Read more
48 reactions Q182
GEN-IAL® QuickGEN Dekkera bruxellensis quantitative high

Qualitative and quantitative detection of Dekkera bruxellensis in wine and grape must.

Read more
48 reactions (freeze-dried, tubes incl. Lytikase and IC-DNA). Q371
GEN-IAL® QuickGEN Yeast Zygosaccharomyces bailii high

Detection of Zygosaccharomyces bailii in wine and grape must by real-time PCR.

Read more
48 reactions (tubes incl. Lyticase and IC-DNA) Q561
GEN-IAL® Simplex® Easy Wine

Fast and optimal DNA-extraction from microorganisms in wine samples and grape must.

Read more
100 Extractions Q300
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