Microbiology

Test kits for the detection of microbiological contamination

  1. Home
  2. /
  3. Analytes
  4. /
  5. Microbiology

Analytes

Microbiology

Food contaminated with microorganisms such as bacteria, yeasts, molds or viruses may pose a risk to the consumer. In addition to the detection of pathogenic microorganisms, it is important to monitor typical spoilage organisms in order to reduce losses during production.

Microorganisms are creatures of microscopically small size, mainly organized as single-cell organisms, while some of them may occur multicellularly. They can be found everywhere in the natural environment (water, soil, air, etc.). Microorganisms may naturally be present in foods or on the surfaces of foods (e.g. fresh vegetables) or added as contamination during the manufacturing process of food products (e.g. insufficient hygiene in meat processing).

In food industry, it can be distinguished between different categories of microbiological contamination:

Pathogenic microorganisms are either not allowed at all to occur in the foodstuff or they are limited to a specified number of cells per gram food. These limits differ to the respective food matrix. (For more information please visit the DGHM Website.) If limits are exceeded, this might have tremendous consequences for consumer’s health. Therefore, a zero tolerance per 25 g of food sample is predicted for most of all common pathogens (SalmonellaCampylobacterE. coli, etc.).

In general, you can differentiate between two methods of microbiological testing: food testing and hygiene monitoring / cleaning control.

Detection of microorganisms in the production environment

Microbiological tests for cleaning and hygiene control detect general residues of foods on insufficient cleaned surfaces in the production environment. These residues of nutrients are the basis for growth of microorganisms.

Colony counting in foods or on surfaces can be done using the ready-to-use Compact Dry plates. Compact Dry plates are available for 13 different parameters of analysis. General method base of examinations with Compact Dry is the cultivation of microorganisms on standard nutrient media. The test principle is based on specific chromogenic substrates which are converted into colored products by the metabolism of microorganisms. Beside examination of diluted food samples, Compact Dry is also suited for swab samples from surfaces or incubation of membrane filter samples from liquids.

Residues of foods on surfaces can be detected using protein tests (RIDA®CHECK) or ATP measurements (LuciPac™Pen, LuciPac™ A3 Surface). Determination of proteins roughly describes the stage of contamination by color change of the colorimetric detection reagent. However, the relative amount of ATP (Adenosintriphosphat) or AMP (Adenosinmonophosphat) respectively can be measured very precisely. A special bioluminescence reaction which requires the presence of ATP or AMP is recorded in a special luminometer instrument (Lumitester SMART). Both systems are suited for consistent control of efficiency of cleaning procedures. These controls ensure maintenance of general industrial hygiene. Surfaces which are hygienically clean can reduce the risk of product contaminations.

Compact DryRIDA®CHECKLumitester SMART
Ready-to-use nutrient platesProtein testATP / AMP measurement
Microbial count on surfaces and in diluted food samplesSwab test for the detection of food residues on surfacesDetection of food residues on surfaces
Easy and safeRapid and reliable methodRapid and convenient using LuciPac®Pen and LuciPac™ A3 Surface with integrated swab

Compact Dry

Compact Dry is an easy test method for counting microorganisms. Learn more in our product flyer.

Detection of microorganisms in foods

Toxic residues of bacteria in food and beverage samples can be analyzed with test kits for bacterial toxins. Common test formats for microbial food testing are ELISA assaysreal-time PCR tests, nutrient plates and agar plates.

For detection of pathogenic bacteria, immunological based methods (ELISA) are available. RIDASCREEN® assays are based on the sandwich technology, in which the antigen (bacterial surface protein) will be captured in the well of a microplate and detected by adding a second antibody which is marked with a special detection enzyme. The immunological detection requires suitable pre-enrichment procedures depending on the kind of pathogen bacteria.

The detection of bacterial toxins is possible directly from food samples (RIDASCREEN® SET Total and RIDASCREEN® SET A,B,C,D,E) or is used for indirect detection of toxin producing pathogenic bacteria subsequent to appropriate pre-enrichment.

The identification of pathogenic bacteria and viruses can be performed with molecular biological systems, e.g. the highly specific real-time PCR kits of the SureFast® PATHOGEN line. The modular system consists of optimized DNA preparation via spin filter columns and the commonly used TaqMan hydrolysis probe technology linked with FAM dyes for detection. It can be performed with all commonly available types of real-time PCR thermo cyclers. The internal amplification control is integrated into all PLUS kits. The appropriate choice of amplification control dye depends on the cycler type where the reaction will be applied to.

RIDASCREEN®SureFast®Compact Dry
Immunological test (ELISA)Molecular-biological test (real-time PCR)Ready-to-use nutrient plates
For the detection of bacteria and bacterial toxinsFor the detection of bacteria and virusesMicrobial count on surfaces and in diluted food samples
Rapid and reliable methodHighly specific and fast; suitable for all standard PCR thermal cyclersEasy and safe

SureFast® Microbiology

SureFast® real-time PCR tests are efficient and highly specific molecular biological tests for detecting microorganisms. Learn more in our product flyer.

Product portfolio

ProductDescriptionNo. of tests/amountArt. No.
RIDASCREEN® SET Total (96 tests)

RIDASCREEN® SET Total is a sandwich enzyme immunoassay for the combined detection of Staphylococcus aureus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures.

Read more
Microtiter plate with 96 wells (12 strips with 8 breakable wells each),
96 determinations.
R4105
RIDASCREEN® SET A,B,C,D,E

RIDASCREEN® SET A,B,C,D,E is a sandwich enzyme immunoassay for the identification of Staphylococcus enterotoxins A, B, C, D and E in fluid and solid foods as well as in bacterial cultures. Based on its sensitivity the RIDASCREEN® SET A,B,C,D,E test …

Read more
Microtiter plate with 96 wells (12 strips with 8 wells each),
one strip is necessary for analysis of one sample,
12 tests in total.
R4101
load more
ProductDescriptionNo. of tests/amountArt. No.
RIDA®CHECK

RIDA®CHECK is a rapid swab test for monitoring the efficiency of surface cleaning procedures in the production environment. The test is intended to be used as a support for hygiene monitoring as well as general protein screening in allergen …

Read more
R1091: 50 swabs for 100 determinations
R1092: 20 swabs for 40 determinations
R1091 / R1092
load more
ProductDescriptionNo. of tests/amountArt. No.
Compact Dry YMR

Usage of Compact Dry YMR (rapid) is a simple, safe and fast test procedure for determination and quantification of yeasts and molds in foods or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special 50 …

Read more
HS9801: 100 nutrient plates,
HS9802: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9801 / HS9802
Compact Dry AQ

Usage of Compact Dry AQ is a simple and safe test procedure for determination and quantification of heterotrophic water bacteria from water samples (drinking and ultrapure water). The ready-to-use plates consist of a special 50 mm diameter petri dish …

Read more
HS9541: 100 nutrient plates,
HS9542: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9541 / HS9542
Compact Dry ETC

Usage of Compact Dry ETC is a simple and safe test procedure for determination and quantification of Enterococcus in foods and water samples. The ready-to-use plates consist of a special 50 mm diameter petri dish containing a detection specific …

Read more
HS9461: 100 nutrient plates,
HS9462: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9461 / HS9462
Compact Dry BC

Usage of Compact Dry BC is a simple and safe test procedure for determination and quantification of Bacillus cereus in foods or raw materials. The ready-to-use plates consist of a special 50 mm diameter petri dish containing a detection specific …

Read more
HS9721: 100 nutrient plates,
HS9722: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9721 / HS9722
Compact Dry X-SA

Usage of Compact Dry X-SA is a simple and safe test procedure for determination and quantification of Staphylococcus aureus in foods, cosmetics or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special …

Read more
HS9621: 100 nutrient plates,
HS9622: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS9621 / HS9622
Compact Dry YM

Usage of Compact Dry YM is a simple and safe test procedure for determination and quantification of yeasts and molds in foods or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special 50 mm diameter …

Read more
HS8801: 100 nutrient plates,
HS8802: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS8801 / HS8802
Compact Dry TC

Usage of Compact Dry TC is a simple and safe test procedure for determination and quantification of aerobic mesophilic count in foods, cosmetics or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a …

Read more
HS8771: 100 nutrient plates,
HS8772: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS8771 / HS8772
Compact Dry ETB

Usage of Compact Dry ETB is a simple and safe test procedure for determination and quantification of Enterobacteria in foods, cosmetics or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special 50 mm …

Read more
HS9431: 100 nutrient plates,
HS9432: 40 nutrient plates (HS9432),
four plates are packed in one aluminum bag.
HS9431 / HS9432
Compact Dry EC

Usage of Compact Dry EC is a simple and safe test procedure for determination and quantification of coliform bacteria and E. coli in foods, cosmetics, water or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist …

Read more
HS8781: 100 nutrient plates,
HS8782: 40 nutrient plates (HS8782),
four plates are packed in one aluminum bag.
HS8781 / HS8782
Compact Dry CF

Usage of Compact Dry CF is a simple and safe test procedure for determination and quantification of coliform bacteria in foods, cosmetics or raw materials – as well as pharmaceutical raw materials. The ready-to-use plates consist of a special 50 mm …

Read more
HS8791: 100 nutrient plates,
HS8792: 40 nutrient plates,
four plates are packed in one aluminum bag.
HS8791 / HS8792
load more
ProductDescriptionNo. of tests/amountArt. No.
SureFast® Listeria 3plex ONE

The SureFast® Listeria 3plex ONE can be applied for the fast and simple isolation and differentiation of Listeria spp. and Listeria monocytogenes in different types of food. The method consists of the following three steps: 1) Cultural enrichment 2) …

Read more
100 reactions F5217
SureFast® STEC 4plex ONE

The SureFast® STEC 4plex ONE can be applied for the fast and simple isolation, detection and differentiation of specific DNA sequences of the Escherichia coli virulence factors stx1 (subtype a-d), stx2 (subtype a-g), eae and the Escherichia coli …

Read more
100 reactions F5265
TANBead Maelstrom™ 4800

The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to …

Read more
Up to 48 samples ZMAL48
SureFast® SARS-CoV-2 PLUS

The SureFast® SARS-CoV-2 PLUS is a real-time RT-PCR for the direct, qualitative detection of novel coronavirus (SARS-CoV-2) RNA. Each reaction contains an Internal Control RNA (ICR, consisting of MS2-bacteriophage) as an internal control of sample …

Read more
100 reactions F7110
SureFast® Mag PREP Food

The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 8 Autostage or Maelstrom 4800. Standardization and harmonization of …

Read more
96 preparations F1060
SureFast® Salmonella ONE

Salmonella spp. detection with real-time PCR Two in one – Salmonella test kit with included DNA preparation The kit is intended to be used for the fast and simple isolation and detection of Salmonella DNA from enrichment cultures. Each reaction …

Read more
100 reactions F5211
SureFast® Salmonella PLUS

The SureFast® Salmonella PLUS is a real-time PCR for the direct, qualitative detection of a specific DNA sequence of Salmonella spp.. The SureFast® Salmonella PLUS real-time PCR kit has been validated and certified in combination with the …

Read more
100 reactions F5111
load more