EuroProxima AHD
Art. No.: 5091AHD
Product info
Intended use
EuroProxima AHDis a competitive enzyme immunoassay for quantitative analysis of AHD in urine, tissue, milk, egg, honey, shrimps and fish.
General Information
The nitrofurans are a group of synthetic broad-spectrum antibiotics, which have been widely and effectively used for the prevention and treatment of gastrointestinal infections caused by Escherichia coli, Salmonella spp., Mycoplasma spp., Coccidiaspp., coliforms and other protozoa in animal production and aquaculture. Moreover, nitrofurans have been employed as growth promoters in livestock.
The four major nitrofurans are furazolidone, furaltadone, nitrofurantoin and nitrofurazone. They have been banned in the EU for use as veterinary drugs, due to their toxic an suspected carcinogenic and mutagenic properties (Commission Regulation 1442/95). In 2003 a definitive MRPL (Minimum Required Performance Limit) was set at 1 ng/g (ppb) in the EU for all four of the above mentioned nitrofurans in poultry and aquaculture products (Commission Decision 2003/181/EC).
Various studies have demonstrated that the nitrofuran parent molecules are rapidly metabolised by animals and that their in vivo stability is not longer than a few hours. As a result, persistent protein-bound residues are formed. Unlike the parent molecules,these protein-bound metabolites are stable and persistent in the body. It is possible to free these residues from proteins by acid hydrolysis. Testing for the presence of nitrofurans is thus equivalent to testing for the presence of a part of the parent molecule, i.e. the free residue. Nitrofuran residues are found after administration of furaltadone (3-amino-5-morpholinomethyl-2-oxazolidinone = AMOZ), furazolidone (3-amino-2-oxazolidinone = AOZ), nitrofurantoin (1-aminohydantoin = AHD) and nitrofurazone (semicarbazide = SEM).
Note: For the determination of AMOZ, AOZ and SEM we refer to the EuroProxima ELISAs 5091AMOZ, 5091AOZ and 5091SEM, respectively.
| Specifications | |
|---|---|
| Art. No | 5091AHD |
| Test format | Microtiter plate with 96 wells (12 strips with 8 wells each). |
| Sample preparation | For mentioned matrices fast and efficient extraction methods are included in the kit description. |
| Incubation time | 45 min |
| LOD (Detection Limit) | urine (0.2 μg/L (ppb)), tissue (0.2 μg/kg), milk (0.2 μg/L), egg (0.2 μg/kg), honey (0.5 μg/kg), shrimps (0.2 μg/kg), fish (0.2 μg/kg) |
| Validated matrices | Urine, tissue, milk, egg, honey, shrimps and fish. |
| Detected analyte | AHD in urine, tissue, milk, egg, honey, shrimps and fish. |
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
| Files | |
|---|---|
| Instructions | English |
| MSDS | 5091AHD.zip (English) |