EuroProxima Beta-Agonist Fast
Art. No.: 5061BAGFc
Product info
Intended use
EuroProxima Beta-Agonist Fast is a competitive enzyme immunoassay for quantitative analysis of β-agonists on the presence of urine, faeces, kidney, bile and plasma, liver, tissue, milk and feed.
General Information
β-Agonistic drugs are used in human healthcare for the treatment of for instance asthma. In recent years, it has been established that a number of β-agonistic drugs may have repartitioning effects in meat producing animals. The flow of nutrients is apparently shifted from adipose tissue towards muscle tissue. The result is an improved lean meat deposition and higher production efficiency. The use of β-agonists as feed additives is not permitted in the European Community. Nevertheless, there have been reported several incidences of the use of clenbuterol, salbutamol, mabuterol, mapenterol and bromobuterol in a number of European countries. Urine is still the most frequently analysed sample material, however, other sample materials are used for different reasons. In farmhouses, urine, faeces, hair and feed can be sampled. Sampling of faeces is much easier and faster than sampling of urine, and the residue levels for β-agonists are comparable. At slaughter, edible tissues (liver, kidney and muscle) can be sampled next to body fluids (plasma, urine and bile) and eye samples. Bile is one of the most suitable sample material for the control on misuse of anabolic steroids and can be preferred for the control of both steroids and β-agonists. Plasma samples are frequently used for pharmacokinetic studies. Clenbuterol accumulates in the choroid/pigmented retinal epithelium tissue of the bovine eye and even after a withdrawal period of 140 days, clenbuterol can still be detected which makes the material extremely suitable for the control on misuse of clenbuterol. Clenbuterol is permitted for use as a drug in pregnant cows. For this reason a method for screening for the presence of clenbuterol in milk samples is included in this manual as well. A number of studies have been published using the β-agonist-ELISA kit of EuroProxima.
| Specifications | |
|---|---|
| Art. No | 5061BAGFc |
| Test format | Microtiter plate with 96 wells (12 strips with 8 wells each). |
| Sample preparation | For mentioned matrices fast and efficient extraction methods are included in the kit description. |
| Incubation time | 1 h |
| LOD (Detection Limit) | Urine (0.15 μg/L (ppb)), faeces, kidney, bile and plasma (0.25 μg/kg), liver (0.1 μg/kg), tissue (0.1 μg/kg), milk (0.04 μg/L), feed (1.0 μg/kg) |
| Validated matrices | Urine, faeces, kidney, bile and plasma, liver, tissue, milk and feed. |
| Detected analyte | β-agonists on the presence of urine, faeces, kidney, bile and plasma, liver, tissue, milk and feed. |
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
| Files | |
|---|---|
| Instructions | English |
| MSDS | 5061BAGFc.zip (English) |