Art. No.: RBRP27
A qualitative screening card for detection of aflatoxin B1 at various levels in food and feed commodities.
The kit is based on monoclonal antibody technology which has the advantage of being highly specific and sensitive while the test format is rapid and simple to perform. The screening procedure is intended to serve as an indicator of the presence of toxins at various screening levels according to international legislation.
The toxin is extracted from the sample, filtered and passed through a clean-up column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less than the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card. The assay takes approximately 10 minutes to perform.
- The technology used in the test kit is highly specific and sensitive.
- A further advantage is the easy application of the test.
- Low expenditure of time.
|Test format||10 cards containing 20 tests and controls|
|Sample preparation||A representative sample should be obtained by following an officially recognised sampling procedure. Sample is extracted using solvent, filtered and passed through an solid phase extraction column (supplied in the test kit) before applying to the test card.|
|Incubation time||The total assay time takes approximately 10 minutes to perform.|
|LOD (Detection Limit)||Qualitative detection at various screening levels|
|Available application notes|
Methods are available for all matrices covered by legislation as well as additional commodities. Please contact your local R-Biopharm distributor for further information.