A qualitative screening card for detection of aflatoxin B1 at various levels in food and feed commodities.
The kit is based on monoclonal antibody technology which has the advantage of being highly specific and sensitive while the test format is rapid and simple to perform. The screening procedure is intended to serve as an indicator of the presence of toxins at various screening levels according to international legislation.
The toxin is extracted from the sample, filtered and passed through a clean-up column before being diluted and applied to the card. The conjugate is applied to the membrane and unbound conjugate is then removed by washing. A colourless substrate is added and the card is incubated for five minutes. Finally a stop solution is applied to the membrane. A purple spot must appear at the control site to indicate that the test is valid. A purple spot at the sample site shows that the contamination is less that the cut off value of the card. No colour at the sample site indicates contamination at a higher level than the cut off of the card.
|Test format||10 cards containing 20 tests and controls.|
|Incubation time||5 minutes.|
|Detection limit||Varies depending on dilution used.|