SureFood® GMO ID 4plex Soya II

Art. No.: S2162

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SureFood® GMO ID 4plex Soya II

Art. No.: S2162

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Intended use

SureFood® GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modified soya DNA sequences listed as below:

  • Roundup Ready Soya (RR Soya) (OECD unique identifier MON-Ø4Ø32-6)
  • Roundup Ready 2 Yield Soya (RR2Y Soya) (OECD unique identifier MON-89788-1)
  • A2704-12 Soya (OECD unique identifier ACS-GMØØ5-3)
  • A5547-127 Soya (OECD unique identifier ACS-GMØØ6-4)

The detections and differentiation are performed according to the validated method of the European Commission respectively official collection of detection methods of §64 German food law.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Mx3005P, Applied Biosystems 7500, Bio-Rad CFX96, R-Biopharm RIDA®CYCLER, Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer and Qiagen Rotor-Gene Q.

Accessories

Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com.

Specifications
Art. NoS2162
Test format100 reactions
Sample preparationFor DNA-preparation of raw material the use of SureFood® PREP Basic (Art. No. S1052), SureFast® Mag PREP Food (Art. No. F1060) and for highly processed food and feed the use of SureFood® PREP Advanced (Art. No. S1053) is recommended. SureFood® PREP Add On (Art. No. S1055) is intended to be used for the extraction of DNA from raw materials as well as processed food and feed with sample weight of 2 g. It is used in conjunction with the SureFood® PREP Basic.
LOD (Detection Limit)The SureFood® GMO ID 4plex Soya II real-time PCR has a limit of detection of < 5 DNA copies. This is equivalent to approx. 0.01 % of unprocessed soybean.