Intended use:

The test detects the genetically construct P35S:BAR. The LibertyLink 601 (LL601 – OECD unique identifier BCS-OSØØ3-7) Rice and the LibertyLink 62 (LL62 – OECD unique identifier ACS-OSØØ2-5) Rice contains this genetically construct but it is also present in other GMOs as StarLink Corn and Bt176 Corn.

The detection of P35S:BAR is according to the validated method of the European Commission (“Report on the verification of a construct-specific detection method for identification of Rice GM-events containing P35S:BAR using a real-time PCR assay” from the Joint Research Centre, the Community Reference Laboratory). Additionally the kit contains a reference PCR system for the detection of Rice.


General Information:

The real-time PCR assay can be used with established real-time PCR instruments (Roche LightCycler®, Rotor-Gene Q, ABI PRISM, Eppendorf realplex, BioRad CFX96, Agilent MxSeries etc.).


Article NumbersS2022
Test format 100 reactions, whereas 50 reactions are used for detection of the reference gene.
Sample preparation The real-time PCR kit can be used in combination with the SureFood® PREP Basic or SureFood® PREP Advanced Kit. The use of SureFood® PREP Advanced kit protocol 1 (preparation of 100 mg of the homogenized sample with 1.3 ml Lysis Buffer) increases the DNA yield. This ensures the required limit of detection of 0.01 % in unprocessed Rice grain.
LOD (Detection limit) The P35S:BAR PCR has a limit of detection of ≤ 5 DNA copies. This is equivalent to approx. 0.01 % for unprocessed Rice grain. The assay limit of detection depends on sample matrix, processing grade, DNA preparation and DNA content.
Cross reactivity

none known

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