Organismos modificados genéticamente
Soluciones innovadoras para la detección de GMO en los alimentos
Organismos modificados genéticamente
La producción mundial de alimentos modificados genéticamente (por ejemplo, soja y maíz) ha aumentado de forma continua desde el comienzo de la década de los 90.
Desde el 1 de septiembre de 1998, todos los productos alimentarios que contienen organismos modificados genéticamente (GMO) se deben especificar en la etiqueta. De acuerdo con la normativa del Consejo de Ministros de la Unión Europea (UE), normativa para etiquetado de nuevos alimentos: N.º de decisión 1139/98/CE. Además, la directiva de la UE 1829/2003, que entró en vigor en abril de 2004, determina un valor umbral de etiquetado del 0,9 % en la UE para alimentos y piensos.
Detección cualitativa de GMO
De acuerdo con la normativa nacional o regional, se aplican unos niveles de tolerancia cero a los eventos de GMO no aprobados. De ahí que, por el momento, solo se consideren de interés los análisis cualitativos en estos productos. El límite de detección se encuentra en el 0,01 %, dependiendo de la matriz y la aplicación.
La regulación CE 619/2011 es una normativa especial para piensos. Siempre que se cumplan ciertas condiciones (caducidad de la aprobación de la UE, aprobación en el país de origen, disponibilidad de ensayos y material de referencia, etc.), se aplicará un valor umbral técnico del 0,1 % para eventos de GMO no aprobados. Esto es particularmente importante en las importaciones de maíz y soja con y sin GMO, no siempre libres de contaminación.
Detección cuantitativa de GMO
En los eventos de GMO aprobados respectivamente, existe la pregunta cuantitativa de si el producto supera los valores umbral definidos o los valores de etiquetado obligatorios.
En Europa, se aplica un valor del 0,9 % al evento de GMO en la matriz vegetal respectiva. Por debajo de este valor, no es necesario declarar productos alimentarios con contaminaciones casuales o técnicamente inevitables de eventos de GMO (CE 2829/2003 y EC2830/CE). La cuantificación se consigue a través de la cuantificación respectiva de un gen específico de GMO y un gen específico de matriz vegetal de acuerdo con una curva de calibración diferente en ambos casos, y la conversión al número de copias de ADN de GMO/matriz vegetal. Al multiplicar esta unidad adimensional por 100 se obtiene como resultado el valor de porcentaje. La curva de calibración se obtiene mediante la dilución de un estándar de ADN y, de acuerdo con un material de GMO definido, también se puede especificar de forma precisa con un factor de corrección experimental.
Cribado de GMO
El cribado de secuencias de genes extrínsecas a la planta infiltradas en ella durante el desarrollo del GMO proporciona una evidencia eficiente de la presencia de posibles GMO.
Las secuencias genéticas utilizadas habitualmente son las siguientes:
This promoter, made from the cauliflower mosaic virus, is the most frequently used element. Plant samples that are only 35S positive can also be contaminated with the naturally occurring virus. False positive results can be ruled out by using the virus-specific CaMV (S2027) test.
The Nopalin Synthase Terminator (NOS) from Agrobacterium tumefaciens is often but not exclusively used in combination with 35S.
In lieu of 35S, the figwort mosaic virus promoter can also be used in GMOs.
The gene for phosphinotricin-N-acetyltransferase from Streptomyces hygroscopicus can enable a resistance against the herbicide glufosinate that is available under several different brand names.
This sequence denotes the transition from chloroplast transit peptide to 5-enolpyruvylshikimate-3-phosphate synthase from Arabidopsis thaliana and Agrobacterium spp. It enables a resistance against the herbicide glyphosate.
Estos y otros parámetros se pueden combinar de forma modular o bien utilizar por separado. La eficiencia de la preparación del ADN se puede verificar mediante el kit PLANT Plus (S2049) específico de la planta.
SureFood® es un sistema modular basado en los 3 componentes principales, amplificación y detección. Cada componente consta de varios módulos. Todos los módulos son compatibles. Esto convierte a SureFood® es un sistema extremadamente flexible que se puede configurar fácilmente para diversas aplicaciones específicas del usuario. SureFood® está disponible como producto y también como servicio.
Resumen del producto SureFood® GMO
Encontrará un amplio resumen de los kits de ensayo SureFood® GMO en nuestro folleto gratuito.
Product portfolio
| Product | Descripción | No. of tests/amount | Art. No. |
|---|---|---|---|
| SureFood® GMO ID 4plex Soya III |
The SureFood® GMO ID 4plex Soya III is a multiplex real-time PCR kit for the direct, qualitative detection and differentiation of following specific genetically modified soya DNA sequences: – FG72 soya (OECD unique identifier MST-FGØ72-2) … Lee más |
100 reactions | S2164 |
| TANBead Maelstrom™ 4800 |
The TANBead Maelstrom™ 4800 (Taiwan Advanced Nanotech) is a fully automated operating instrument for nucleic acid purification and isolation using magnetic beads and rods. The instrument uses the TANBead patented Maelstrom Spin Mixing Technology to … Lee más |
Up to 48 samples | ZMAL48 |
| SureFood® GMO QUANT MON810 Corn |
The test detects the relative quantitative MON810 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON810 Corn (OECD unique identifier MON-ØØ81Ø-6) and the other one specific for Corn (the reference gene). The … Lee más |
2 x 50 reactions | S2019 |
| SureFood® GMO ID DAS-40278-9 Corn |
The SureFood® GMO ID DAS-40278-9 Corn is a real-time PCR kit for the direct, qualitative detection of a specific genetically modified corn DNA sequence. Therefore the kit contains an event specific real-time PCR system for the DAS-40278-9 corn (OECD … Lee más |
100 reactions | S2140 |
| SureFast® Mag PREP Food |
The kit is intended to be used for isolation of animal and plant DNA from food and feed as well as bacterial DNA from bacterial culture enrichments with the TANBead® Maelstrom 4800. Standardization and harmonization of methods Reduction of errors … Lee más |
96 preparations | F1060 |
| SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/ CTP2:CP4 EPSPS |
The SureFood® GMO SCREEN 4plex BAR/PAT/CryIAb/IAc/CTP2:CP4 EPSPS is a multiplex real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified DNA – sequences. … Lee más |
100 reactions | S2128 |
| SureFood® GMO ID 4plex Corn I |
The SureFood® GMO ID 4plex Corn I is a multiplex real-time PCR for the direct, qualitative detection and differentiation of following specific genetically modified corn DNA sequences: FAM channel: MON810 corn (OECD unique identifier … Lee más |
100 reactions | S2170 |
| SureFood® GMO ID 4plex Soya II |
SureFood® GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modified soya DNA sequences listed as below: Roundup Ready Soya (RR Soya) (OECD unique identifier … Lee más |
100 reactions | S2162 |
| SureFood® GMO ID 4plex Canola II |
The multiplex real-time PCR test detects the following DNA-sequences of genetically modified canola: – FAM channel: MON88302 canola (OECD unique identifier MON-883Ø2-9) – VIC channel: Canola – ROX channel: DP73496 canola (OECD … Lee más |
100 reactions | S2167 |
| SureFood® GMO ID 4plex Canola I |
The multiplex test detects the following DNA sequences of genetically modified canola: FAM channel: MS8 canola (OECD unique identifier ACS-BNØØ5-8) ROX channel: GT73 canola (OECD unique identifier MON-ØØØ73-7) Cy5 channel: T45 canola (OECD … Lee más |
100 reactions | S2166 |
| SureFood® GMO ID 4plex Soya I |
The multiplex test SureFood® GMO ID 4plex Soya I detects the following DNA-sequences of soya: FAM-channel: MON87708 Soja (OECD Bezeichnung MON-877Ø8-9) FAM-channel: CV127 Soja (OECD Bezeichnung BPS-CV127-9) VIC-channel: DP305423 Soja (OECD … Lee más |
100 reactions | S2161 |
| SureFood® GMO SCREEN 4plex BAR/NPTII/PAT/CTP2:CP4 EPSPS |
This kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. The multiplex test detects the following DNA-sequences: Phosphinothricin-Acetyltransferase gene (BAR) from Streptomyces hygroscopius … Lee más |
100 reactions | S2127 |
| SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC |
The SureFood® GMO Plant 4plex Corn/Soya/Canola+IAC is a multiplex real-time PCR for the direct, qualitative detection and differentiation of following specific corn/soya/canola DNA sequences. Lee más |
100 reactions | S2158 |
| SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton |
The SureFood® GMO Plant 4plex Corn/Soya/Canola/Cotton is a multiplex real-time PCR for the direct, qualitative detection and differentiation of following specific corn/soya/canola/cotton DNA sequences. Lee más |
100 reactions | S2156 |
| SureFood® GMO QUANT GT73 Canola |
SureFood® GMO QUANT GT73 Canola is a real-time PCR for the detection of the relative GT73 canola DNA amount to the total canola DNA content. Therefore, the kit contains two PCR systems, one for detection of a GT73 canola specific gene (OECD unique … Lee más |
2 x 50 reactions | S2061 |
| SureFood® GMO QUANT MON863 Corn |
SureFood® GMO QUANT MON863 Corn is a real-time PCR for the detection of the relative MON863 corn DNA amount to the total corn DNA content. Therefore, the kit contains two PCR systems, one for detection of a MON863 corn-specific gene (YieldGard™ … Lee más |
2 x 50 reactions | S2051 |
| SureFood® GMO PLANT PLUS |
The test detects DNA of plants qualitatively. Each reaction contains an internal amplification control (IAC). Lee más |
100 reactions | S2049 |
| SureFood® GMO QUANT MIR162 Corn |
The test detects the relative quantitative MIR162-Corn DNA amount. The kit contains two PCR systems, one event-specific for the MIR162-Corn (OECD unique identifier SYN-IR162-4) and the other one specific for Corn (reference system). Lee más |
2 x 50 reactions | S2135 |
| SureFood® GMO SCREEN 4plex 35S/NOS/FMV/IAC |
This multiplex real-time PCR kit can be used for screening of genetically modified organisms (GMOs) in food, feed and seeds. For this purpose, PCR systems for detection of the 35S Cauliflower Mosaic Virus (CaMV) promoter DNA sequence, A. tumefaciens … Lee más |
100 reactions | S2126 |
| SureFood® GMO SCREEN CaMV |
The test detects CaMV DNA. The presence of CaMV DNA indicates an infestation with the cauliflower mosaic virus. Host of the CaMV are Brassicaceae plants. With the virus specific detection system SureFood® GMO CaMV it is possible to verify the … Lee más |
100 reactions | S2027 |
| SureFood® GMO QUANT T25 Corn |
The test detects the relative quantitative T25-Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the T25-Corn (OECD unique identifier ACS-ZMØØ3-2) and the other one specific for Corn (reference system). The … Lee más |
2 x 50 reactions | S2017 |
| SureFood® GMO QUANT RR2Y Soya |
SureFood® GMO QUANT RR2Y Soya is a real-time PCR for the detection of the relative Roundup Ready 2 Yield® Soya DNA amount (RR2Y soya) to the total soya DNA content. Therefore, the kit contains two PCR systems, one for detection of a RR2Y … Lee más |
2×50 reactions | S2029 |
| SureFood® GMO QUANT RoundUp Ready Soya |
SureFood® GMO QUANT Roundup Ready Soya (RR soya) is a real-time PCR for the detection of the relative RR soya DNA amount to the total soya DNA content. Therefore, the kit contains two PCR systems, one for detection of a RR soya specific gene … Lee más |
2×50 reactions | S2014 |
| SureFood® GMO QUANT Bt176 Corn |
The test detects the relative quantitative Bt176-Corn DNA amount. Therefore the kit contains two PCR systems, one construct specific for the Bt176-Corn (OECD unique identifier SYN-EV176-9) and the other one specific for Corn (reference system). … Lee más |
2 x 50 reactions | S2015 |
| SureFood® GMO QUANT Bt11 Corn |
The test detects the relative quantitative Bt11 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the event Bt11 Corn (OECD unique identifier SYN-BTØ11-1) and the other one specific for Corn (the reference gene). The … Lee más |
2 x 50 reactions | S2016 |
| SureFood® GMO QUANT 35S Soya |
SureFood® GMO QUANT 35S Soya is a real-time PCR for the detection of the relative 35S CaMV promoter DNA amount to the total soya DNA content in food, feed and seeds. Therefore, the kit contains two PCR systems, one for detection of a 35S CaMV … Lee más |
2×50 reactions | S2028 |
| SureFood® GMO QUANT 35S Corn |
The test is developed for the relative estimation of the 35S CaMV DNA amount in only Corn containing products. Therefore the kit contains two PCR systems, one specific for the 35S CaMV promoter sequence and the other one specific for Corn (the … Lee más |
2×50 reactions, whereof 50 reactions are used for the quantification of the reference gene. | S2020 |
| SureFood® GMO SCREEN P35S:BAR Rice |
The SureFood® GMO SCREEN P35S:BAR Rice is a real-time PCR for the direct, qualitative detection of a specific P35S:BAR constructDNA sequence of P35S:BAR. The LibertyLink 601 (LL601 – OECD unique identifier BCS-OSØØ3-7) Rice and the … Lee más |
100 reactions, whereas 50 reactions are used for detection of the reference gene. | S2022 |
| SureFood® GMO ID Bt63 Rice |
The SureFood® GMO ID Bt63 Rice is a real-time PCR for the direct, qualitative detection of a specific genetically modified Bt63 rice DNA sequence. Therefore, the kit contains a construct specific real-time PCR system for the Bt63 rice (OECD unique … Lee más |
2 x 50 reactions | S2024 |
