EuroProxima Chloramphenicol
Art. No. 5091CAP
Product info
Intended use
EuroProxima Chloramphenicol is a competitive enzyme immunoassay for the quantitative analysis of chloramphenicol in urine, liver, tissue, milk, feed, egg and honey.
General Information
The use of the broad-spectrum antibiotic chloramphenicol (CAP) was completely banned in the European Union by amending Annex IV of Council Regulation (EEC) 2377/90 with Commission Regulation 1430/94. In 2003, a minimum performance limit (MRPL) for CAP residues in food of animal origin was set at 0.3 μg/kg. This CAP ELISA is suitable for the detection of CAP and its glucuronide (main metabolite in urine). CAP can be detected in urine directly (after dilution in buffer) or after ethyl acetate extraction to increase sensitivity and specificity. Defatted milk samples can also be added directly to the test with a detection limit of 0.1 ng/ml. This detection limit can be reduced tenfold by extraction in ethyl acetate. For eggs and tissue samples, including shrimps, crabs and fish, the ethyl acetate extraction is followed by a purification step with a mixture of iso-octane/trichloromethane or n-hexane, resulting in detection limits of 0.04 to 0.02 ng/g.
| Specifications | |
|---|---|
| Art. No | 5091CAP |
| Test format | Microtiter plate with 96 wells (12 strips with 8 individual wells each). |
| Sample preparation | Fast and efficient methods are included in the test kit description for the matrices mentioned. |
| Incubation time | 1h 30 min |
| LOD (Detection Limit) | Urine (0.01 μg/L (ppb)), liver (0.01 μg/kg), tissue (0.02 μg/kg), milk (0.01 μg/L), animal feed (0.1 μg/kg), egg (0.02 μg/kg), honey (0.02 μg/kg) |
| Validated matrices | Urine, liver, tissue, milk, animal feed, egg and honey. |
| Detected analyte | Chloramphenicol in urine, liver, tissue, milk, animal feed, egg and honey. |
| Evaluation | Microplate photometer (450 nm) |
| Files | |
|---|---|
| Instructions | German/English |
| MSDS | 5091CAP-1.zip (English) |
