SureFood® ALLERGEN Hazelnut
Art. No.: S3602

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SureFood® ALLERGEN Hazelnut

Art. No.: S3602
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Intended use

The real-time PCR test detects DNA of hazelnut (Corylus avellana, Corylus maxima) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control (IAC).

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 (Art. No. S3301) containing 40 mg hazelnut / kg food sample and a detection system including a standard series is required.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of two fluorescence emissions at the channels FAM and VIC/HEX at the same time. The technical verification of instruments was performed on Agilent AriaDx, Applied Biosystems 7500, Bio Molecular Systems MIC, Bio-Rad CFX96, LTF MyGo Pro, Qiagen Rotor-Gene Q, Roche cobas® z 480 Analyzer and Roche LightCycler® 480 II.

Accessories

Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st of January 2024, you can find our documents on the eIFU portal congen.de/information.

Specifications
Art. NoS3602
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 or SureFast® Mag PREP Food (Art. No. F1060) is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN Hazelnut PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
LOQ (Limit of quantification

1 mg / kg using SureFood® PREP Advanced, protocol 1

EvaluationReal-time PCR instrument with two detection channels (510 nm and 580 nm)