Intended use:

The test detects hazelnut DNA (Glycine max) according to directive (EC) 1169/2011 qualitatively and / or quantitatively. Each reaction contains an internal amplification control.

For the quantitative determination the use of the laboratory reference material SureFood® QUANTARD Allergen 40 containing 40 mg hazelnut / kg food sample and a detection system including a standard dilution is required.

For DNA preparation, the use of SureFood® PREP Advanced, protocol 1, is recommended.

 

General information:

The real-time PCR assay can be used with established real-time PCR instruments capable of detection of at least two fluorescence dyes. Internal validation was performed on Roche LightCycler® 480 II, Roche cobas® z 480 Analyzer, Qiagen Rotor-Gene Q, Applied Biosystems 7500, Bio-Rad CFX96, Bio Molecular Systems MIC, LTF MyGo Pro, AriaDx and Agilent Mx3005P.

Accessories:

Specifications
Art. No. S3602
Test format 100 reactions
Sample preparation For DNA-preparation the use of SureFood® PREP Advanced, protocol 1 is recommended.
Detection limit The SureFood® ALLERGEN Hazelnut PCR has a limit of detection of ≤ 0.4 mg / kg. The limit of quantification is 1 mg / kg using SureFood® PREP Advanced, protocol 1. The limit of detection and limit of quantification were determined using the SureFood® QUANTARD Allergen 40 (matrix: corn flour).
The limit of detection of the complete quantitative determination (DNA extraction and real-time PCR) depends on sample matrix, processing grade, DNA-preparation and DNA-content.
Files
InstructionsGerman/English
Similar Products
SureFood® GMO ID 4plex Soya II – NEW
SureFood® GMO ID 4plex Soya...
SureFood® GMO ID 4plex Soya II is a multiplex real-time PCR for the direct, qualitative detection and differentiation of specific genetically modif...
SureFood® Apricot
SureFood® Apricot
The test detects apricot (Prunus armenica) DNA. Each reaction contains an internal amplification control.
SureFood® GMO QUANT MON89034 Corn – NEW
SureFood® GMO QUANT MON8903...
The test detects the relative quantitative MON89034 Corn DNA amount. Therefore the kit contains two PCR systems, one specific for the MON89034 Corn...

Start typing and press Enter to search