Art. No.: R7001

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Art. No.: R7001
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Intended use

RIDASCREEN® Gliadin (Art. No. R7001) is a R5 sandwich enzyme immunoassay based on specific monoclonal antibody to celiac toxic amino acid prolamin sequences to determine gliadin as a measure of gluten in food.

It is applicable for the quantitative measurement of intact gliadin as a measure of gluten in unprocessed and processed matrices from important gluten-free food categories, including rice- and corn-based products, soy, starches, pseudo cereals, legumes, spices, juice, nut nougat crème, cream cheese, pesto, meat, vegetarian meat alternative, cookies, dessert, cake, fish, bread, candies, and potatoes. The sandwich ELISA quantifies intact gliadin from wheat and related proteins from rye and barley. This method is not accurate for quantification of fermented or hydrolyzed gluten.

General Information

Gluten is a mixture of prolamin and glutelin proteins present in wheat, rye and barley. Prolamins from wheat are named gliadins.

The use of gluten in foodstuffs is extremely common because of its useful effects on e.g. texture, moisture retention and flavor. However, gluten intolerance disorders like coeliac disease require a permanent gluten-free diet to avoid clinical symptoms. According to the Codex Alimentarius (CODEX STAN 118/1979) two categories for labeling of dietary food according to the gluten content exist:

1) “Glutenfree” = Food products, containing < 20 mg / kg gluten
2) “Very low gluten” = Food products containing 20 – 100 mg / kg gluten

The threshold of 20 mg / kg has been adopted by many national legislations in many countries. The Méndez method – a combination of R5 ELISA and a special, patented buffer (cocktail) for optimized extraction of gliadin from heat-processed and non-heated food samples – is the Codex Alimentarius recommended method for analysis of gluten in food. It is Codex Alimentarius type I method for analysis of gliadin/gluten. Categorization as type I method defines it as reference method. R-Biopharm is the only R5 ELISA manufacturer who offers the licensed Cocktail (patented) (Art. No. R7006 / R7016) and hence fulfills the requirements of the reference method to 100 %.

Furthermore, RIDASCREEN® Gliadin is in combination with the Cocktail (patented) the only R5 ELISA, which is AOAC Official Method of Analysis (OMA 2012.01). Applicability for analysis of principally all foods is an important requirement to function as Méndez method in the meaning of Codex Alimentarius. The publication of the 3rd international collaborative proficiency study from 2020 describes the preparation of 900 samples of 19 differently processed foods from 16 food categories and the obtained results demonstrating it suitability for gluten detection in gluten-free foods. Accordingly, RIDASCREEN® Gliadin received from the AOAC as first assay worldwide the status as Official Method of Analysis (AOAC OMA 2012.01) “in food” in 2021. A limitation on certain validated food matrices as usual for other AOAC approvals is not present any longer.

Further approvals:

The RIDASCREEN® Gliadin in combination with the Cocktail (patented) is not only Codex Alimentarius type I method and AOAC Official Method of Analysis. It is also revised and approved method of AOAC-RI, AACCI and ICC (see below “Certificates”).

Watch our video tutorials for sample preparation and test procedure with RIDASCREEN® Gliadin – please see below.



Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com.

Art. NoR7001
Test formatMicrotiter plate with 96 wells (12 strips with 8 removable wells each)
Sample preparationHomogenization and extraction
Incubation time1 h 30 min
Room temperature
LOD (Detection Limit)0.5 mg / kg (ppm) Gliadin (mean) or 1 mg / kg (ppm) Gluten
0.06 - 1.24 mg / kg (ppm) Gliadin* (*depending on matrix)
LOQ (Limit of quantification

2.5 mg / kg (ppm) Gliadin or 5 mg / kg (ppm) Gluten

Validated matrices

Rice- and corn-based products, soy, starches, pseudo cereals, legumes, spices, juice, nut nougat crème, cream cheese, pesto, meat, vegetarian meat alternative, cookies, dessert, cake, fish, bread, candies, and potatoes

Detected analyte

QQPFP epitope that occurs in α-/β-, γ-, and ω-gliadins; B-, C-, and γ-hordeins; as well as γ- and ω-secalins from wheat, rye, and barley

Available application notes
  • Allergen swabbing using RIDASCREEN® Allergen ELISAs.
  • RIDASCREEN Gliadin in combination with RIDA® Extraction Solution (colorless) (R7098) – only after validation.
  • Sample preparation for raw materials with ethanol.
  • Sample preparation for polyphenol containing raw materials (e.g. chocolate, cofee, cacao, buckwheat) with fish gelatine and ethanol.
EvaluationMicrotiter plate spectrophotometer (450 nm)