SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

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SureFood® ALLERGEN 4plex Macadamia / Brazil Nut / Pecan + IAC

Art. No.: S3403

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Intended use

The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC is a real-time PCR for the direct, qualitative detection and differentiation of specific macadamia (Macadamia ternifolia), brazil nut (Bertholletia excelsa) and pecan (Carya illinoinensis) DNA sequences according to directive (EC) 1169/2011.

General Information

The real-time PCR assay can be performed with commonly used real-time PCR instruments, equipped for detection of four fluorescence emissions at the channels FAM, VIC/HEX, ROX and Cy5 at the same time. The technical validation of instruments was performed on Agilent Aria Dx, Bio-Rad CFX96 Dx, Roche LightCycler® 480 II and R-Biopharm RIDACYCLER.

Accessories

Specifications
Art. NoS3403
Test format100 reactions
Sample preparationFor DNA-preparation the use of SureFood® PREP Advanced (Art. No. S1053), protocol 1 is recommended.
LOD (Detection Limit)The SureFood® ALLERGEN 4plex Macadamia/Brazil Nut/Pecan + IAC PCR has a limit of detection of ≤ 0.4 mg / kg using SureFood® PREP Advanced, protocol 1.
Cross Reactivity.

For the Pecan real-time PCR system cross reactivity was observed with DNA extracts from shagbark hickory (Carya Ovata).