Usage of Compact Dry X-BC is a simple and safe test procedure for determination and quantification of Bacillus cereus in foods or raw materials. The plates consist of a special 50 mm diameter petri dish containing a detection specific nutrient pad.
The ready-to-use, chromogenic Compact Dry plates are suitable for in-process controls as well as controls of finished products. With the Promedia ST-25 swab tests (Art. No. Z0302, available from R-Biopharm) Compact Dry plates may also be used for surface sampling and therefore applied for quantitative hygiene monitoring even at surfaces which are difficult to reach.
The Gram-positive bacterium Bacillus cereus is able to produce long lasting spores and is commonly found in soil and water. Therefore, it enters the food processing chain relatively easy. B. cereus can spoil foods; furthermore, its emetic and diarrheagenic enterotoxins cause vomiting and diarrhea after consumption. Routine testing procedures for B. cereus are thus recommended.
Compact Dry X-BC plates are incubated at 30° C for 24 hours. Due to the chromogenic substances in the medium, B. cereus grows as green-blue colonies. If bacteria other than Bacillus cereus grow on the plate, they usually produce white colonies. The non-pathogenic organism Bacillus thuringiensis which is a close relative to B. cereus has the same biochemical properties. It can therefore also grow and produce green-blue colonies on Compact Dry X-BC.
Compact Dry X-BC has been approved by MicroVal (certificate No 2011-LR41) and NordVal (certificate No.045) according to ISO 16140. During the studies a broad range of different food matrices has been tested.
|Art. No.||HS9721; HS9722|
|Test format||100 nutrient plates or 40 nutrient plates, respectively - – where four plates are packed in one aluminum bag|
|Incubation time||24 ± 2 h at 30 ± 1°C|