Colorimetric assay for the determination of Oxalic acid in wine, beer, beverages, honey and other foodstuffs.
As an alternative to the “Yellow line” enzymatic kits produced by Roche Diagnostics, R-Biopharm also offers the Enzytec™ Generic line, which includes enzymatic or colorimetric assays. The Enzytec™ Generic line includes 14 different enzymatic kits, which can detect acids and sugars.
Oxalate is oxidized to carbon dioxide and hydrogen peroxide by oxalate oxidase. Hydrogen peroxide reacts in presence of peroxidase (POD) with MBTH (3-methyl-2-benzothiazolinone hydrazone) and DMAB (3-dimethylamino benzoic acid) forming a blue quinone compound. The intensity of color is proportional to the concentration of oxalate in the sample and it is read at 590 nm.
|Test format||1 x 20 ml for 10 determinations|
|Incubation time||15 minutes|
|LOD (Detection limit)||1.5 mg Oxalic acid / L|
|Evaluation||Colorimetric method (590 nm)|
|Certificate of Analysis|