Art. No.: 5121DON
EuroProxima Deoxynivalenol is a competitive enzyme immunoassay for quantitative analysis of deoxynivalenol (DON) in cereals, feed, food, beer and silage.
Deoxynivalenol belongs to the group of trichothecenes produced essentially by a broad range of Fusarium moulds of which F. graminearum and F. culmorum are the
most important. These fungi typically develop during prolonged cool, wet growing and harvest seasons to produce Fusarium head blight in cereal crop. Deoxynivalenol, also known as ‘vomitoxin’, because of its potent emetic effects in swine, is most often associated with Fusarium head blight. Some fungal strains make DON by a 3-
acetylated precursor (mainly in Europe and Asia) and others produce a 15-acetylated precursor (mainly in North and South America). The amount of 3-acetyl-DON and/or
15-acetyl-DON in a sample is approximately 10 % of the amount of DON present. Significant concentrations of DON are frequently detected in wheat, barley, corn and
oats, while lower levels are usually found in rye, sorghum and rice. About 40-50 % of all samples analysed were found positive for DON with average contents of 290 µg/kg (range 2 – 10.000 µg/kg). This assay is suitable for screening purposes on the presence of the mycotoxin DON in food and feed products, but also in liquid samples as beer. For confirmation purposes a previous HPLC isolation in combination with massspectrometry analysis is necessary.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each).|
|Sample preparation||For mentioned matrices fast and efficient extraction methods are included in the kit description.|
|Incubation time||1 h 30 min|
|LOD (Detection Limit)||Cereals (1.5 μg/kg (ppb)), feed (30 μg/kg), food (30 μg/kg), beer (1.5 μg/L), silage (50 μg/kg)|
Cereals, feed, food, beer and silage.
Deoxynivalenol (DON) in cereals, feed, food, beer and silage.
|Evaluation||Microtiter plate spectrophotometer (450 nm)|