RIDASCREEN® Ractopamin is a competitive enzyme immunoassay for the quantitative analysis of ractopamine in urine, meat and liver and for the qualitative analysis of ractopamin glucuronide in urine.
Ractopamine belongs to the group of β-agonists. It is known that β-agonists are suitable for use as performance improvers within the field of livestock production. In particular, ractopamine reduces fat, increases average daily weight gain, and improves feed conversion ratio (FCR) in fattened animals. However, β-agonists have not been authorized as growth promoters in livestock in the European Union (EU). In addition to its lipolytic and anabolic effect, ractopamine has also a relaxing effect on non-striated musculature, on which its therapeutic use as antiasthmatic agent is based. It is possible that ractopamine residues, after use in illegal practice, may lead to a risk for consumers. Concequently, the use of ractopamine has been banned in most countries.
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Sample preparation||urine (direct): without sample preparation|
urine (after hydrolysis): hydrolisation, extraction, centrifugation and evaporation
meat, liver: homogenization, extraction, centrifugation, evaporation and centrifugation
|Incubation time||1 h 30 min|
|Detection limit||urine (inclusive hydrolysis): approx. 700 ng/l (ppt)|
meat: approx. 200 ng/kg (ppt)
liver: approx. 300 ng/kg (ppt)
(corresponding to the standard substance)
Ractopamine (standard substance): 100 %
The anti-ractopamine antibody binds also glucuronidated ractopamine. The specificity of the antibody for ractopamine glucuronide A, B and C in swine urine is approx. 10 – 20 %.
Dobutamine: approx. 4.7 %