RIDASCREEN® Sulfonamide is a competitive enzyme immunoassay for the quantitative analysis of sulfonamides in egg, meat (chicken and pork), fish, shrimp, honey and milk.
Sulfonamides are widely used as feed additives, mainly for fattening of calves and pigs. Combined with inhibitors of dihydrofolate reductase such as trimethoprim, tetroxoprim, or pyrimethamine sulfonamides are also used in veterinary medicine for the treatment of intestinal infections, mastitis, pulmonitis and other (systemic) diseases. Therefore sulfonamide residues may occur in food of animal origin such as meat and milk and were found frequently abroad. According to the EU-Law a maximum residue limit for all substances of the sulfonamide-group of 100 µg/kg in muscle, fat, liver and kidney and of 100 µg/l in milk is valid.
|Test format||Microtiter plate with 96 wells (12 strips with 8 removable wells each)|
|Sample preparation||milk: after 1:5 dilution direct use in the assay|
meat (chicken and pork), egg, fish, shrimp: homogenization, extraction, centrifugation, evaporation and degreasing
honey: extraction, RIDA® C18 column purification and dilution
|Incubation time||1 h 15 min|
|LOD (Detection limit)||meat (chicken), egg: 1.5 µg/kg (ppb)|
meat (pork), fish, shrimp, honey: 2 µg/kg (ppb)
milk: 3.5 µg/l (ppb)
(corresponding to the standard substance)
Sulfamethoxypyridazine: approx. 100 %