RIDASCREEN® Gliadin
Art. No.: R7001
Product info
Intended use
RIDASCREEN® Gliadin (Art. No. R7001) is a sandwich enzyme immunoassay for the quantitative analysis of prolamins from wheat (gliadin), rye (secalin) and barley (hordein) in food. The test is based on a specific monoclonal antibody (R5) against amino acid sequences in prolamins that are toxic to coeliac disease patients.
The RIDASCREEN® Gliadin in combination with the Cocktail (patented) is Codex Alimentarius type I method (CODEX STAN 234-1999) and AOAC Official Method of Analysis (OMA 2012.01). Further international certificates/approvals are:
- AOAC-RI – PTM certification no. 120601
- AACCI – Approved Method 38-50.01
- ICC Standard Method no. 182
It is applicable for the quantitative measurement of intact gliadin as a measure of gluten in unprocessed and processed matrices from important gluten-free food categories. The sandwich ELISA quantifies intact gliadin from wheat and related proteins from rye and barley. This method is not accurate for quantification of fermented or hydrolyzed gluten.
The Méndez method – a combination of R5 ELISA and a special, patented buffer (cocktail) for optimized extraction of gliadin from heat-processed and non-heated food samples – is the Codex Alimentarius recommended method for analysis of gluten in food. The Méndez method is Codex Alimentarius type I method for analysis of gliadin/gluten. Categorization as type I method defines it as reference method. R-Biopharm is the only R5 ELISA manufacturer who offers the licensed Cocktail (patented) (Art. No. R7006 / R7016) and hence fulfills the requirements of the reference method to 100 %.
Furthermore, RIDASCREEN® Gliadin is in combination with the Cocktail (patented) the only R5 ELISA, which is AOAC Official Method of Analysis (OMA 2012.01). Applicability for analysis of principally all foods is an important requirement to function as Méndez method in the meaning of Codex Alimentarius. The publication of the 3rd international collaborative proficiency study from 2020 describes the preparation of 900 samples of 19 differently processed foods from 16 food categories and the obtained results demonstrating it suitability for gluten detection in gluten-free foods. Accordingly, RIDASCREEN® Gliadin received from the AOAC as first assay worldwide the status as Official Method of Analysis (AOAC OMA 2012.01) “in foods” in 2021. A limitation on certain validated food matrices as usual for other AOAC approvals is not present any longer.
General Information
The use of gluten in foodstuffs is extremely common because of its useful effects on e.g. texture, moisture retention and flavor. Gluten is a mixture of prolamin and glutelin proteins present in wheat, rye and barley. Prolamins from wheat are named gliadins. However, gluten intolerance disorders like coeliac disease require a permanent gluten-free diet to avoid clinical symptoms. According to the Codex Alimentarius (CODEX STAN 118/1979) two categories for labeling of dietary food according to the gluten content exist:
1.) “Gluten-free” = Food products, containing < 20 mg / kg gluten
2.) “Very low gluten” = Food products containing 20-100 mg / kg gluten
The threshold of 20 mg / kg has been adopted by many national legislations in many countries. The prolamine content (e.g. gliadin) of gluten is defined as 50 % (CODEX STAN 118-1979).
Benefits:
- Best validated test kit (three international collaborative studies)
- Only R-Biopharm offers Cocktail (patented) which is the official extraction procedure working in accordance to Codex Method (Type I)
Watch our video tutorials for sample preparation and test procedure with RIDASCREEN® Gliadin – please see below.
Patent Marking:
If the R-Biopharm product “Cocktail ECO” is used for the processing of food samples, a sulphite-containing extraction agent is used. Food inspection methods using a sulfite-containing extractant as in this product and/or corresponding detection kits are subject to the following patents of MORINAGA & Co., Ltd.: European Patent EP 2 224 239 B1, Australian Patent AU 2008 330 507 B2, United States Patent US 8 859 212 B2, Japanese Patent JP 5 451 854 B2. The patent holder has granted R-Biopharm AG a license to use, and sell products that employ, said protected technology in the above-mentioned territories.
Accessories
- Cocktail (patented)
- Cocktail ECO
- RIDA® Extraction Solution (colorless)
- Set of 3 processed Gliadin Assay Controls
Videos
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Dear customers,
we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.
| Specifications | |
|---|---|
| Art. No | R7001 |
| Test format | Microtiter plate with 96 wells (12 strips with 8 removable wells each) |
| Sample preparation | Homogenization and extraction |
| Incubation time | 1 h 30 min Room temperature |
| LOD (Detection Limit) | 0.5 mg / kg (ppm) Gliadin (mean) or 1 mg / kg (ppm) Gluten 0.06 - 1.24 mg / kg (ppm) Gliadin* (*depending on matrix) |
| LOQ (Limit of quantification | 2.5 mg / kg (ppm) Gliadin or 5 mg / kg (ppm) Gluten |
| Validated matrices | Rice- and corn-based products, soy, starches, pseudo cereals, legumes, spices, juice, nut nougat crème, cream cheese, pesto, meat, vegetarian meat alternative, cookies, dessert, cake, fish, bread, candies, and potatoes |
| Detected analyte | • prolamins from wheat, rye and barley (spelt, kamut) |
| Available application notes |
|
| Evaluation | Microtiter plate spectrophotometer (450 nm) |
| Approvals | • AOAC-OMA 2012.01 "Final Action" ("in foods" claim) • AOAC-RI (120601) • ACCI 38.50.01 • Codex Alimentarius Methode (Typ I) • ICC |
