Intended use:
A simple membrane test for the rapid detection of non-durum wheat adulteration of durum wheat (semolina).
General Information:
The kit uses a monoclonal antibody which is specific for the protein friabilin which is present in non-durum wheats but not in durum wheat.
| Article Numbers | RBRP10 |
|---|---|
| Test format | Reagents and membrane strips suitable for 40 analysis. |
| Sample preparation | Flour samples are extracted to release any friabilin protein. The extract is then absorbed onto the membrane, and the strip is saturated with a blocking agent. An enzyme labelled monoclonal antibody specific to the protein is then added. If non-durum wheat is present in the sample then a complex will be formed between the extracted friabilin protein and the enzyme labelled antibody. After washing the strip, a clear coloured staining reagent is added which forms a blue / purple coloured deposit in the presence of the friabilin /antibody enzyme complex. The degree of blue / purple colour with the sample is proportional to the percentage of non-durum wheat present. No colour developed indicates 100 % durum wheat (i.e. no adulteration). |
| Incubation time | The total assay time takes approximately 35 minutes to perform. |
| LOD (Detection limit) | Detects 3 % non-durum wheat. |
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