Intended use:

A simple membrane test for the rapid detection of non-durum wheat adulteration of durum wheat (semolina).

General Information:

The kit uses a monoclonal antibody which is specific for the protein friabilin which is present in non-durum wheats but not in durum wheat.

Specifications
Article NumbersRBRP10
Test format Reagents and membrane strips suitable for 40 analysis.
Sample preparation Flour samples are extracted to release any friabilin protein. The extract is then absorbed onto the membrane, and the strip is saturated with a blocking agent. An enzyme labelled monoclonal antibody specific to the protein is then added. If non-durum wheat is present in the sample then a complex will be formed between the extracted friabilin protein and the enzyme labelled antibody. After washing the strip, a clear coloured staining reagent is added which forms a blue / purple coloured deposit in the presence of the friabilin /antibody enzyme complex. The degree of blue / purple colour with the sample is proportional to the percentage of non-durum wheat present. No colour developed indicates 100 % durum wheat (i.e. no adulteration).
Incubation time The total assay time takes approximately 35 minutes to perform.
LOD (Detection limit) Detects 3 % non-durum wheat.
Files
Instructions
MSDS
Similar products
DUROTEST® S
DUROTEST® S
A simple membrane test for the rapid detection of non-durum wheat adulteration of durum wheat (semolina).
RIDA®CUBE SCAN
RIDA®CUBE SCAN
The RIDA®CUBE SCAN is a photometric system that allows biochemistry testing, covering all enzymatic and colorimetric assays for the detection of or...
RIDA®SMART APP
RIDA®SMART APP
The RIDA®SMART APP is a software application for the quantification of RIDA®QUICK mycotoxin lateral flow tests. The RIDA®SMART APP is a complete...

Product support

Questions? Tap into our team’s expertise. We’re here to support you and your business throughout the testing process to ensure your success.

Start typing and press Enter to search

surefood-pcr