Art. No.: RBRP88 / RBRP88B

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Art. No.: RBRP88 / RBRP88B

Intended use

Immunoaffinity columns for use in conjunction with an HPLC or LC-MS/MS system for detection of vitamin B12 in a wide range of commodities.

Novel immunoaffinity columns with built-in reservoir for detection of vitamin B12 in a wide of range commodities, developed in conjunction with Nestlé Research Centre, Switzerland.

General Information

The procedure is based on monoclonal antibody technology, which makes the test highly specific, sensitive, rapid and simple to perform. The columns contain a gel suspension of monoclonal antibody specific to the vitamin of interest. Following extraction of the vitamin the sample extract is diluted with buffer and filtered before being passed slowly through the immunoaffinity column. Any vitamin which is present in the sample is retained by the antibody within the gel suspension. The column is washed to remove any unbound material and the vitamin is then released from the column following elution with solvent. The eluate is collected, evaporated and reconstituted prior to analysis by HPLC or LC-MS/MS.


  • AOAC Official Final Action method 2014.02.
  • Unique format immunoaffinity column.
  • Uses monoclonal antibody.
  • Extraction uses buffer and enzymes.
  • Can be used with pigmented samples.
  • Can be stored at 21 – 25 °C.
  • The columns are suitable for testing a wide range of commodities.



Dear customers,

we now provide the documents for our products in an electronic format which include the Instructions for Use (IFU), Safety Data Sheets (SDS), Application notes and the Certificate of Analysis (CoA). For batches placed on the market after 1st June 2023, you can find our documents on the eIFU portal eifu.r-biopharm.com/rbr.

Art. NoRBRP88 / RBRP88B
Test formatRBRP88 = 10 immunoaffinity columns with 10 ml format.
RBRP88B = 50 immunoaffinity columns with 10 ml format.
Sample preparationSample is extracted using buffer and enzymes, filtered and diluted ready for applying to the immunoaffinity column. The LGE column is a unique format with no other immunoaffinity column having this design. This immunoaffinity allows the sample extract to mix directly with the antibody gel within the column ensuring that the best recoveries and lowest % RSD are achieved.
Incubation timeSample is incubated for 30 mins at 37 °C followed by 30 mins at 100 °C.
LOD (Detection Limit)Information provided is typical LOD obtained by R-Biopharm. Please note that this is dependant on method, LC conditions and equipment used.

Cyanocobalamin 5 ng / ml
Detected analyte


Available application notes

Methods are available for a range of commodities. Please contact your local R-Biopharm distributor for further information.

EvaluationHPLC or LC-MS/MS system
ApprovalsProduct referenced in AOAC Official Final Action Method 2014.02