Intended use:

Quantitative determination of high molecular weight ß-Glucan from barley in malt-mash and wort (EBC 4.16.3, MEBAK, in combination with sample extraction method EBC 4.5.1. The assay does not involve any enzyme, the reaction is a chemical / colorimetric reaction (therefore it belongs ot the product line Enzytec™ Color).

Two test-kits are available for respectively 40 tests (Art. No. E3500) and 160 tests (Art. No. E3550).

General Information:

The determination of the ß-Glucan concentration is important for the malt and beer industry.

The test has been developed by the company NovaBiotec – Dr. Fechter GmbH and production has been transferred to R-Biopharm AG. The raw materials and the production procedures for the original GlucaTest® Rapid assay (NovaBiotec) and the new Enzytec Color Gluca Test kits are identical. Only the packaging has been slightly changed in order to fit to the new production facilities.


  • Easy: Principle of photometric test; without special sample preparation; without additional laboratory equipment.
  • Fast: Results after 30 minutes reaction time & quasi-online process control is possible.
  • Favorable: Savings compared to conventional methods of analysis.
  • Reliable: High correlation between photometric and fluorometric methods.

Article NumbersE3500 / E3550
Test format • GlucaTest S125 (E3500) = 40 tests
(1 x 125 ml solution A + calibrator set),
• GlucaTest L500 (E3550) = 160 tests
(4 x 125 ml solution A + calibrator set)
Sample preparation The method for sample preparation has been described under EBC method 4.5.1 (Extract of malt: Congress mash). It is mandatory to prepare the samples according to this method if the test is used according to EBC 4.16.3.
Incubation time 30 minutes
Validated matrices

barley in malt-mash and wort

Detection range 100 - 500 mg/L ß-Glucan
Detected analyte


Evaluation Colorimetric assay (550 nm)
sdsE3500_-_Enzytec™_Color_GlucaTest®_S125_DE.pdf (German)
sdsE3500_-_Enzytec™_Color_GlucaTest®_S125_GB.pdf (English)
sdsE3500_-_Enzytec™_Color_GlucaTest®_S125_FR.pdf (French)
sdsE3500_-_Enzytec™_Color_GlucaTest®_S125_IT.pdf (Italian)
sdsE3500_-_Enzytec™_Color_GlucaTest®_S125_ES.pdf (Spanish)
sdse3500_-_enzytec_color_glucatest_s125_bg.pdf (Bulgarian)
sdse3500_-_enzytec_color_glucatest_s125_cz.pdf (Czech)
sdse3500_-_enzytec_color_glucatest_s125_dk.pdf (Danish)
sdse3500_-_enzytec_color_glucatest_s125_ee.pdf (Estonian)
sdse3500_-_enzytec_color_glucatest_s125_fi.pdf (Finnish)
sdse3500_-_enzytec_color_glucatest_s125_gr.pdf (Greek)
sdse3500_-_enzytec_color_glucatest_s125_hr.pdf (Croatian)
sdse3500_-_enzytec_color_glucatest_s125_hu.pdf (Hungarian)
sdse3500_-_enzytec_color_glucatest_s125_lt.pdf (Lithuanian)
sdse3500_-_enzytec_color_glucatest_s125_lv.pdf (Latvian)
sdse3500_-_enzytec_color_glucatest_s125_nl.pdf (Dutch)
sdse3500_-_enzytec_color_glucatest_s125_no.pdf (Norwegian)
sdse3500_-_enzytec_color_glucatest_s125_pl.pdf (Polish)
sdse3500_-_enzytec_color_glucatest_s125_pt.pdf (Portuguese)
sdse3500_-_enzytec_color_glucatest_s125_ro.pdf (Romanian)
sdse3500_-_enzytec_color_glucatest_s125_se.pdf (Swedish)
sdse3500_-_enzytec_color_glucatest_s125_si.pdf (Slovenian)
sdse3500_-_enzytec_color_glucatest_s125_sk.pdf (Slovakian)
sdse3500_-_enzytec_color_glucatest_s125_tr.pdf (Turkish)
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