Enzytec™ Color Iron

Art. No.: E2300

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Product info

Enzytec™ Color Iron

Art. No.: E2300

Intended use

This test is a colorimetric assay used mainly in wine laboratories for determination of the Iron (Fe) concentration in wine and other food samples. The assay does not involve any enzyme, the reaction is a chemical / colorimetric reaction (therefore it belongs ot the product line Enzytec™ Color).

General Information

The determination of the Iron concentration is important for the wine industry and belongs to the range of colorimetric tests performed in analysis of wine as the determination of Copper, SO2-free, SO2-total and tartaric acid. These tests are based on the use of chromogens, without any enzyme involved in the reaction.

The test principle is that Iron is dissociated from proteins in particular conditions of ionic strength, and reduced to a bivalent state through ascorbic acid. It will then react with the chromogenic reagent FERENE-S and build a stable complex, giving a blue colour which is proportional to the concentration of Iron in the sample and is measured at 582 nm.


  • Easy: Principle of photometric test; without special sample preparation; without additional laboratory equipment.
  • Fast: Results after 15 minutes reaction time.
  • Favorable: Savings compared to conventional methods of analysis.
  • Reliable: High correlation between photometric and fluorometric methods.
Dear customers,

we have started to provide the documents for our products in an electronic format. These are the Instructions for Use (IFU), the Safety Data Sheets (SDS) and the Certificate of Analysis (CoA). For batches placed on the market after 01 January 2024, you can find our documents on the eIFU portal eifu.r-biopharm.com/food.

Art. NoE2300
Test format4 x 100 ml (200 assays)
Sample preparationWine samples are tested directly
Incubation time15 minutes
Validated matrices


Detected analyte


Available application notes


EvaluationColorimetric assay (582 nm)