A competitive enzyme immunoassay for screening and quantitative analysis of corticosteroids in various matrices.
The Corticosteroid ELISA is a competitive ELISA for the detection of various corticosteroids in milk, urine, tissue and feed samples. Within the EU Maximum Residue Limits (MRL’s) for dexamethasone, betamethasone, prednisolone and methylprednisolone are established in milk, muscle (fat), kidney and liver. The MRL for dexamethasone in muscle is 0.75 μg/kg.
The Corticosteroid ELISA is a competitive enzyme immunoassay based on specific antibody sites.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For milk, urine, muscle, liver and feed fast and efficient extraction methods are included in the kit manual.|
|Incubation time||90 minutes|
|LOD (Detection limit)||Milk 0.2 ppb|
Urine 3 ppb
Muscle 0.2 ppb
Liver 1 ppb
Feed 0.6 ppb
Flumethasone 125%, Dexamethasone 100%, Betamethasone 60%, Triamcinolone 33%, Prednisolone 21%, Dianabol 8%, Methylprednisolone 3%