A competitive enzyme immunoassay for screening and quantitative analysis of Lincomycin in various matrices.
Lincomycin is a narrow spectrum antibiotic derived from Streptomyces lincolnensis. It belongs to the lincosamide group which also includes pirlimycin and clindamycin. The EU has set maximum residue limits (MRLs) for lincomycin in different foods of animal origin: 50 μg/kg for fat/eggs, 100 μg/kg for muscle, 150 μg/kg for milk, 500 μg/kg for liver and 1500 μg/kg for kidney. Although in the EU no MRLs have been established for antibiotics in honey, some countries including Belgium, Hungary, Switzerland and the United Kingdom have set Action Limits for lincomycin, which vary from 10 to 50 μg/kg.
The Lincomycin ELISA is a competitive enzyme immunoassay based on specific antibodies directed against lincomycin.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For milk, tissue, liver, honey and egg fast and efficient extraction methods are included in the kit manual.|
|Incubation time||90 minutes|
|Detection limit||Milk 45 ppb|
Tissue 41 ppb
Liver 100 ppb
Honey 7 ppb
Egg 20 ppb
Lincomycin 100%, Clindamycin <0.1%