A competitive enzyme immunoassay for screening and quantitative analysis of erythromycin in various matrices.
Erythromycin is an antibiotic with a broad-spectrum activity, which belongs to the group of macrolides. Maximum Residue Limits (MRLs) for erythromycin for all food producing species are established in Europe: for tissue at 200 ppb, milk at 40 ppb and eggs at 150 ppb. The screening for tylosin and erythromycin is an important tool for control in the honey industry. The use of antibiotics in apiculture is forbidden. Therefore, no MRLs have been set for honey. Nevertheless, Recommended Concentrations (RCs) have been published by the European Union Reference Laboratories. For erythromycin the RC is 20 ppb.
The Erythromycin ELISA is a competitive enzyme immunoassay based on antibodies directed against erythromycin.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For milk, honey, egg, shrimps, fish, liver and urine fast and efficient extraction methods are included in the kit manual.|
|Incubation time||90 minutes|
|LOD (Detection limit)||Milk 4 ppb|
Honey 10 ppb
Egg 10 ppb
Shrimps 10 ppb
Fish 10 ppb
Liver 10 ppb
Urine 4 ppb
Erythromycin 100%, Erythromycin ethylsuccinate 12%, Tylosin 0%, Spiramycin 0%