A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines in various matrices.
The most commonly used tetracyclines in veterinary medicine are tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC). In many countries in the world legislation has become in force to control the use of OTC in aquacultural products like salmon, pangasius and shrimps. Furthermore, the use of OTC in the honey industry has to be controlled.
|Test format||Microtiter plate with 96 wells (12 strips with 8 wells each)|
|Sample preparation||For honey and shrimps/fish fast and efficient methods are included in the kit manual.|
|Incubation time||1 h 30 min|
|LOD (Detection limit)||Honey 5 µg/kg (ppb), shrimps/fish 2 µg/kg|
Honey and shrimp/fish.
|Detected analyte||Oxytetracyclines in honey and shrimp/fish.|
|Evaluation||Microtiter plate spectrophotometer (450 nm)|