A competitive enzyme immunoassay for screening and quantitative analysis of oxytetracyclines.
Tetracyclines are a group of antibiotics derived from Streptomyces spp. with a broad-spectrum activity.The most commonly used tetracyclines in veterinary medicine are tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC). In many countries in the world legislation has become in force to control the use of OTC in aquacultural products like salmon, pangasius and shrimps. Furthermore, the use of OTC in the honey industry has to be controlled.
The Oxytetracycline ELISA is a competitive enzyme immunoassay based on antibodies directed against oxytetracycline.
|Test format||Microtiter plate, 96 wells.|
|Sample preparation||For honey and shrimps/fish fast and efficient methods are included in the kit manual.|
|Incubation time||90 minutes|
|LOD (Detection limit)||Honey 5 ppb|
Shrimps/fish 2 ppb
Oxytetracycline 100%, Tetracycline 133%, Chlortetracycline 138%, Doxycycline 54%